离子液体-加速溶剂萃取-高效液相色谱法测定蜜饯中的有机酸类防腐剂  

Analysis of Organic Acid Preservatives in Glace Fruits by High Performance Liquid Chromatography Combined with Ionic Liquid Based Accelerated Solvent Extraction

作　　者：范云场[1,2] 张社利[3] 陈梅兰[4] 申屠超[4] 朱岩[1] 

机构地区：[1]浙江大学西溪校区化学系,杭州310028 [2]河南理工大学物理化学学院,焦作454003 [3]焦作师范高等专科学校生化系,焦作454000 [4]浙江树人大学生环学院,杭州310015

出　　处：《分析化学》2010年第12期1785-1788,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.20775070;J0830413);浙江省自然科学基金(Nos.R4080124;Y4090104);浙江省钱江人才计划(No.2008R10028);河南理工大学基金(Nos.B2010-14;09-3-054;09-3-036)资助项目

摘　　要：以离子液体氯化1-辛基-3-甲基咪唑盐([Omim]Cl)的水溶液为萃取剂,采用加速溶剂萃取结合高效液相色谱法测定了蜜饯中苯甲酸、山梨酸、肉桂酸等有机酸类防腐剂,优化了加速溶剂萃取实验参数,最佳萃取条件为:离子液体浓度为0.1mol/L,萃取时间为5min,萃取温度为80℃。在最佳条件下,有机酸类防腐剂的检出限为0.4～27.7μg/L。将本方法用于蜜饯样品的检测,回收率为78.2%～113.9%。实验结果表明:离子液体-加速溶剂萃取法快速、高效。The combination of accelerated solvent extraction(ASE) using ionic liquid(IL) 1-octyl-3-methylimidazolium chloride([Omim]Cl) aqueous solution as extractant with high performance liquid chromatography(HPLC) has been developed for the analysis of organic acid preservatives such as benzoic acids,sorbic acid and cinnamic acids in glace fruits.Parameters related to extraction of the organic acid preservatives were optimized.The results indicated that the the optimal extraction conditions were 0.1 mol/L of IL,5 min of extraction time and 80 ℃ of extraction temperature,respectively.Under the optimal conditions,the detection limits(LOD) were in the range of 0.4-27.7 μg/L.The proposed method was applied to extract the organic acid preservatives from different glace fruit samples and the recoveries were between 78.2% and 113.9%.The experimental results demonstrate that the IL based ASE method is fast and highly effective.

关 键 词：离子液体 加速溶剂萃取 防腐剂 高效液相色谱 

分 类 号：TS255.41[轻工技术与工程—农产品加工及贮藏工程]