廿烷五烯酸对人肺癌A-549细胞株的凋亡作用及细胞周期的影响  

Effects of the eicosapentaenoic acid on apoptosis and cell cycle of A-549 human lung cancer cell line

作　　者：安京华[1] 李香善[2] 李星[1] 石俊[1] 

机构地区：[1]延边大学附属医院SICU,吉林延吉133000 [2]延边大学附属医院中心实验室,吉林延吉133000

出　　处：《延边大学医学学报》2010年第4期246-248,共3页Journal of Medical Science Yanbian University

基　　金：国家自然科学基金资助项目(30560152);吉林省教育厅自然科学重点研究计划项目

摘　　要：[目的]观察廿烷五烯酸诱导人肺癌A-549细胞株的凋亡作用及其对A-549细胞周期的影响.[方法]用人肺癌A-549进行体外培养,分为对照组和小、中、大剂量廿烷五烯酸组,分别给予蒸馏水及15,30,60 mg/L廿烷五烯酸,培养48 h,观察细胞变化.对照组加入培养液500μL,实验步骤相同.应用FCM进行定量检测细胞凋亡并作细胞周期分析.[结果]对照组细胞培养48 h,凋亡率仅为0.14%±0.05%,未见亚二倍体峰;用15,30,60 mg/L廿烷五烯酸处理48 h时,细胞出现凋亡峰,凋亡率分别为10.50%±0.56%,19.87%±1.34%,30.12%±1.78%,不同质量浓度凋亡率间差异有统计学意义,廿烷五烯酸质量浓度在15～60 mg/L时,G0/G1期和G2/M期细胞百分比下降,S期细胞百分比上升,阻滞周期于S期.[结论]体外实验中,廿烷五烯酸通过干扰细胞周期抑制人肺癌A-549细胞的生长,主要阻滞于S期;抑制肿瘤细胞增殖可能是廿烷五烯酸抗肺癌作用的机制之一.OBJECTIVE To observe the effects of inducing apoptosis and cell cycle on A-549 human lung cancer cell line induced by the eicosapentaenoic acid(EPA).METHODSA-549 cell line was cultured in vitro,and were divided into control group and small,middle and large dosage of EPA groups.The control group was added with 500μL of culture solution.The A-549 cells were cultured for 48h,and apoptic rate of A-549 cells were detected by FCM,and its cell cycles were analyzed.RESULTSAfter culturing the A-549 cells in control group,the apoptic rate was only 0.14%±0.05%,there was no hypodiploid peak,but in experiment groups treated with 15,30,60mg/L of EPA,the apoptotic peaks were appeared,and the apoptic rate was 10.50%±0.56%,19.87%±1.34%,30.12%±1.78%,respectively,and was statisticlly significant difference in different concentration.At treated with 15—60mg/L of EPA,the cell percentage decreased in G0/G1 and G2/M phase and increased in S phase,and the cell cycle was blocked in S phase.CONCLUSIONThe EPA inhibites A-549 lung cancer cells growth in vitro through interfering with cell cycle,mainly arrested in S phase and can inhibit the tumor cell growth that may be one of the mechanisms of EPA on treating lung cancer.

关 键 词：烯酸 人肺癌 细胞株 凋亡作用 细胞周期抑制 cancer CELL line human CELL cycle 对照组 凋亡率 质量浓度 抑制肿瘤细胞增殖 细胞培养 细胞周期分析 亚二倍体峰 统计学意义 抗肺癌作用 阻滞 细胞凋亡 细胞变化 

分 类 号：R[医药卫生]