炎消热清的镇痛作用及其机理研究  

Analgesic roles of aspirin eugenol ester and its mechanisms

作　　者：李剑勇[1] 于远光[1,2] 王棋文[3] 杨亚军[1] 魏小娟[1] 周旭正[1] 牛建荣[1] 李冰[1] 张继瑜[1] 叶得河[2] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所中国农业科学院新兽药工程重点实验室,甘肃兰州730050 [2]甘肃农业大学动物医学院,甘肃兰州730070 [3]毕节学院草地生态研究所,贵州毕节551700

出　　处：《畜牧与兽医》2010年第10期20-24,共5页Animal Husbandry & Veterinary Medicine

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(BRF060403);甘肃省支撑计划项目(0804NKCA074)

摘　　要：为了研究炎消热清(AEE)的镇痛作用及其可能的机制,采用热板法和扭体法考察AEE的镇痛效果,并观察热刺激下二乙基二硫代氨基甲酸钠(DDC)、氟哌啶醇(Hal)、L-色氨酸(L-Trp)、赛庚啶(CYP)等药物对AEE镇痛作用的影响,用紫外分光光度法测定了热刺激所致疼痛小鼠全脑中的前列腺素E2(PGE2)含量。结果表明,AEE可明显减少醋酸扭体试验中小鼠的扭体次数,降低热刺激小鼠疼痛反应;可显著降低热刺激所致疼痛小鼠全脑中PGE2的含量和二乙基二硫代氨基甲酸钠对痛觉的敏感度,与氟哌啶醇之间存在明显的协同效应,与L-色氨酸和赛庚啶之间拮抗效应显著。说明AEE具有明显的镇痛作用,镇痛机制可能与其降低小鼠全脑中的PGE2含量以及增加去甲肾上腺素(NE)的含量、阻断多巴胺(DA)受体有关。The analgesic roles of aspirin eugenol ester(AEE) were investigated by using Writhing test and Hot plate test,while the influence of some drugs,including Diethyldithiocarbamate(DDC),haloperidol(Hal),L-tryptophan(L-Trp) and cyproheptadine(Cyp),on the analgesic effects under heat stimulation were examined.Also,the contents of PGE2 in the whole brain of mice were measured by ultraviolet spectrophotometry.The result showed that both the writhing induced by acetic acid and the paining by thermal stimulation in mice were significantly eased by AEE;the contents of PGE2 in the whole brain of mice were decreased;the algesa induced by DDC was inhibited;a combined effect between Hal and AEE existed,while L-Trp/CYP had significant antagonism to AEE.It suggested that AEE has an obvious analgesic effect which may be related to decreasing the production of the PGE2 in the whole brain of rats,raising the contents of norepinephrine(NE) and blocking-up the dopamine(DA) receptor.

关 键 词：镇痛作用 机理研究 二硫代氨基甲酸钠 热刺激 小鼠 紫外分光光度法测定 含量 氟哌啶醇 AEE 疼痛反应 色氨酸 赛庚啶 全脑 去甲肾上腺素 二乙基 醋酸扭体试验 PGE2 镇痛效果 镇痛机制 协同效应 

分 类 号：S8[农业科学—畜牧兽医]