大豆异黄酮干预对乳腺癌中ERK1、ERK2和c-Fos表达的影响及意义  

Interference of the expression of ERK1,ERK2,and c-Fos in breast cancer by soybean isoflavones and its significance

作　　者：侯建峰[1] 冯笑山[1] 杨晓鹤[2] 米建强[1] 刑鲁奇[1] 王公平[1] 张广平[1] 高社干[1] 马志坤[2] 郝曙光[1] 段瑞华[1] 陈强[1] 茹艺[1] 

机构地区：[1]河南科技大学第一附属医院肿瘤科,洛阳471003 [2]美国俄克拉荷马大学病理中心,俄克拉荷马73104

出　　处：《肿瘤》2010年第9期744-747,共4页Tumor

基　　金：河南省医学科技攻关项目[编号:200703082]

摘　　要：目的:观察大豆异黄酮(soybean isoflavones)干预MMTV-erbB-2转基因小鼠,对乳腺癌组织中细胞外信号调节激酶1/2(extracellular signal regulated kinase 1/2,ERK1/2)和c-Fos蛋白表达的影响及意义。方法:选择鼠龄3周的健康MMTV-erbB-2转基因雌性小鼠100只,随机分为4组(早期干预组、晚期干预组、全程干预组和对照组),观察各组小鼠乳腺癌的发病率和潜伏期,采用免疫组织化学法检测各组小鼠乳腺癌组织及正常乳腺组织中ERK1、ERK2和c-Fos蛋白的表达并进行统计学分析。结果:早期干预组、晚期干预组、全程干预组和对照组雌性MMTV-erbB-2转基因小鼠乳腺癌的发病率分别为48%、28%、20%和92%;与对照组比较,早期干预组、晚期干预组和全程干预组发病率均降低(P<0.01);全程干预组发病率低于早期干预组(P<0.05),晚期干预组与早期干预组和全程干预组比较,发病率差异均无统计学意义;各组小鼠乳腺癌潜伏期分别为(47.67±9.25)、(47.29±14.13)、(51.8±6.69)和(49.91±8.07)周,各组间差异均无统计学意义。各组小鼠的肿瘤直径比较差异无统计学意义(P>0.05);ERK1、ERK2和c-Fos在乳腺癌组织中的阳性表达率分别为71.6%、68.7%和61.2%,在小鼠正常乳腺组织中阳性表达率分别为27.7%、19.1%和12.8%,差异有统计学意义(P<0.01);与对照组相比,早期干预组、晚期干预组和全程干预组乳腺癌组织ERK1、ERK2和c-Fos阳性表达均有所降低,但差异无统计学意义(P>0.05)。结论:大豆异黄酮对MMTV-erbB-2转基因雌鼠乳腺癌的发生具有一定的预防作用,丝裂原活化蛋白激酶(mitogen activated protein kinase,MAPK)信号转导通路在乳腺癌的发生、发展过程中可能起着重要作用。Objective:To investigate the effects of soybean isoflavones on the expressions of extracellular signal regulated kinase 1/2(ERK1/2) and c-Fos in breast cancer of MMTV-erbB-2 transgenic mice and its significance.Methods:One hundred 3-week old healthy MMTV-erbB-2 transgenic female mice were divided into four groups: early intervention group,advanced intervention group,whole-course intervention group and control group.The incidence rate and the latent period of breast cancer were observed.The expression of ERK1,ERK2 and c-Fos proteins in normal breast tissues and breast cancer tissues were determined using immunohistochemical(SP) method.The statistical difference was analyzed.Results:The incidence rates were 48%,28%,20%,and 92% for early intervention group,advanced intervention group,whole-course intervention group,and control group,respectively.The latent periods were(47.67±9.25) weeks,(47.29±14.13) weeks,(51.8±6.69) weeks,and(49.91±8.07)weeks,respectively.Compared with the control group,the incidence rates were decreased in the other three groups(P<0.01).The incidence rate of whole-course intervention group was lower than that in early intervention group(P<0.05).There was no statistical difference in the incidence rate between early intervention group and advanced intervention group(P>0.05) and between advanced intervention group and whole-course intervention group(P>0.05).There was no significant difference in the latent period of breast cancer among the four groups(P>0.05).And the tumor diameter had no significant difference among the four groups(P>0.05).The positive rates of ERK1,ERK2 and c-Fos in breast cancer tissue were 71.6%,68.7%,and 61.2%,respectively,while the positive rates of these proteins in normal breast tissues were 27.7%,19.1%,and 12.8%,respectively(P<0.01).Compared with the control group,the positive rates of these proteins in breast cancer tissues were decreased in intervention group,advanced intervention group,and whole-course intervention group.But the difference was not significant(P>0.05

关 键 词：大豆异黄酮 小鼠乳腺癌 ERK1 ERK2 C-FOS表达 significance breast cancer 统计学意义 早期干预 乳腺癌组织 发病率 转基因小鼠 阳性表达率 对照组 组间差异 细胞外信号调节激酶 丝裂原活化蛋白激酶 C-FOS蛋白表达 乳腺组织 免疫组织化学法 

分 类 号：R73[医药卫生—肿瘤]