Src激酶抑制剂PP2对人胃癌细胞生物学行为的影响  

The effect of Src kinase inhibitor PP2 on the biological behaviors of human gastric carcinoma SGC-7901 cells

作　　者：于虹[1] 怀娜[1] 马秀梅[2] 

机构地区：[1]内蒙古医学院研究生院,呼和浩特010059 [2]内蒙古医学院病理学中心,呼和浩特010059

出　　处：《肿瘤》2010年第10期822-826,共5页Tumor

基　　金：国家自然科学基金资助项目(编号:30660065);教育部春晖计划资助项目(编号:Z2007-1-01003);博士科研启动金资助项目(编号:NY2006BQ006)

摘　　要：目的:探讨Src激酶抑制剂PP2对人胃癌SGC-7901细胞生长、迁移、侵袭和诱导血管形成等生物学行为的影响。方法:通过Western印迹法检测PP2作用对SGC-7901细胞中Src激酶活化的影响;分别采用MTT法、划痕实验、Transwell小室法和体外诱导血管形成分析实验观察PP2对SGC-7901细胞生长、迁移、基质侵袭和诱导血管形成等生物学行为的影响。结果:MTT法检测结果提示,15μmol/L PP2作用人胃癌SGC-7901细胞12 h时能明显抑制细胞的增殖;5和10μmol/L PP2均能抑制SGC-7901细胞的迁移、侵袭和诱导血管的形成,且其抑制能力随PP2浓度的增加而逐渐增强。结论:Src激酶抑制剂PP2具有抑制人胃癌SGC-7901细胞生长、迁移、侵袭和诱导血管形成的能力,可能对胃癌具有一定的治疗效果。Objective:To investigate the effect of Src kinase inhibitor PP2 on the growth,migration,invasion,and angiogenesis capabilities of human gastric carcinoma cell line SGC-7901.Methods:Western blotting was used to detect the effect of Src inhibitor PP2 on Src activation.MTT assay,wound-healing assay,Transwell chamber,and angiogenesis in vitro assay were performed to detect the effects of Src inhibitor PP2 on the growth,migration,invasion and angiogenesis of human gastric carcinoma cell line SGC-7901,respectively.Results:MTT assay showed that the proliferation of human gastric carcinoma SGC-7901 cells was significantly inhibited after co-culture with 15 μmol/L PP2 for 12 h.PP2 inhibited the migration,invasion,and angiogenesis of human gastric carcinoma cells at 5 and 10 μmol/L in a concentration-dependent manner.Conclusion:Src inhibitor PP2 inhibited the growth,migration,invasion and angiogenesis capabilities of human gastric carcinoma cell line SGC-7901 in vitro.Src inhibitor may be effective in the treatment of gastric carcinoma.

关 键 词：SRC激酶 抑制剂 人胃癌 细胞生物学行为 cells human gastric carcinoma SGC-7901细胞 血管形成 体外诱导 侵袭 迁移 WESTERN印迹法 Transwell小室 生长 细胞的增殖 MTT法 逐渐增强 治疗效果 抑制能力 形成分析 

分 类 号：R73[医药卫生—肿瘤]