桔青霉素人工抗原合成及偶联比测定  

Synthesis of Citrinin Artificial Antigen and Its Coupling Ratio Determination

作　　者：李娇[1,2] 钟理[1] 田世民[2] 金涌[2] 崔艳芬[3] 任香夫[2] 祁淑红[2] 刘畅[2] 

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]中国检科院综合检测中心,北京100123 [3]天津泰达园林建设有限公司,天津300457

出　　处：《安徽农业科学》2010年第36期20737-20739,共3页Journal of Anhui Agricultural Sciences

基　　金：国家863基金项目(2007AA10Z426-1)

摘　　要：[目的]建立一种快速准确测定人工抗原偶联比的方法。[方法]通过载体蛋白BSA合成桔青霉素人工抗原,用SDS-PAGE电泳法、紫外吸收法、质谱法对其进行鉴定。[结果]经过3种方法的鉴定,桔青霉素人工抗原偶联成功,紫外吸收法测得其偶联比为8.4,质谱法测得其偶联比为6。[结论]对3种供试方法比较发现,质谱法能够快速鉴定人工抗原而且能够准确测定人工抗原的偶联比。该研究为桔青霉素克隆抗体的制备及建立桔青霉素的酶联免疫检测方法奠定了基础。[Objective]This study aimed to establish a detection method that could rapidly and accurately identify the coupling ratio of artificial antigen.[Method] Citrinin artificial antigen was synthesized by BSA and identified by SDS-PAGE electrophoresis,UV absorption and mass spectrometry respectively.[Result] After three methods of identification,citrinin artificial antigen was coupled successfully.Coupling ratio was 8.4 by UV absorption while 6 by mass spectrometry.[Conclusion] The comparison of three experiment...

关 键 词：桔青霉素 人工抗原 偶联比 质谱 

分 类 号：S432.4[农业科学—植物病理学]