山东地区AS患者HLA-B27基因多态性分析  

HLA-B27 polymorphism in AS patients in Shandong

作　　者：李钦伟[1] 毛永鑫[1] 李文超[1] 陈立辉 

机构地区：[1]山东省血液中心,济南250015 [2]山东省省立医院,济南250001

出　　处：《医学检验与临床》2006年第1期36-37,共2页Medical Laboratory Science and Clinics

摘　　要：目的凋查HLA-B27等位基因在山东地区的分布情况,研究亚犁水平的HLA-B27等位基因与AS的相关性.方法应用序列特异性引物聚合酶链反应(PCR-SSP)技术检测山东地区HLA-B27阳性AS患者中B2701～25的等位基因.结果在36例HLA-B27阳性的AS患者中,只检测到B*2704和B*2705两种业型等位基因,其中B*2704阳性13例,B*2705阳性23例,构成比分别是36.11%(13/36),63.89%(23/36).结论山东地区AS患者HLA-B27,等位基因以B*2704和B*2705为主,且B*2705最多见;不同地区、不同人群的AS患者,HLA-B27各亚型分布频率存在差异性.Objective To investigate the distribution of HLA-B27 alleles in Shandong area and study the association between HLA-B27 alleles and AS at subtype level.Methods All subtypes B~*2701～25 were detected by PCR-SSP among 36 HLA-B27 positive patients with AS.Results Only two subtypes B~*2704 and B~*2705 were detected among 36 HLA-B27 positive patients with AS.13 patients were B~*2704 positive and 23 were B~*2705 positive.The constitutive ratio were 36,11%13/36) and 63.89%23/36)respectively.Conclusions HLA-B~*2704 and B~*2705 were the major alleles among patients with AS in Shandong area,and B~*2705 was more common;There were differences of the distribution frequency of HLA-B27 subtypes in paticnts with AS among different population and area groups.

关 键 词：山东 地区 患者 等位基因 引物聚合酶链反应 阳性 序列特异性 技术检测 分布情况 分布频率 不同人群 相关性 构成比 差异性 种业 应用 亚型 方法 凋查 

分 类 号：R593.23[医药卫生—内科学]