低温处理成纤维细胞复苏过程延迟再水化的初步研究  

The preliminary study of delayed rehydration of normal human fibroblast after freezing and thawing

作　　者：官浩[1] 贾赤宇[2] 孟庆延[3] 何飞[4] 陈壁[1] 张万福[1] 

机构地区：[1]第四军医大学西京医院烧伤整形中心,西安710032 [2]解放军总医院第一附属医院全军烧伤研究所,北京100037 [3]沈阳军医总医院烧伤科,沈阳110000 [4]吉林大学哲学社会学院社会心理系,吉林132000

出　　处：《感染．炎症．修复》2006年第1期36-38,65,共4页Infection Inflammation Repair

摘　　要：目的:实低温处理成纤维细胞复苏过程存在延迟再水化现象。方法:-10℃处理成纤维细胞后20℃复温,培养1d后离心收集上清漂浮细胞培养,观察脱水漂浮细胞再次贴壁、检测细胞细胞贴壁率、分裂指数、用噻唑蓝(MTT)法确定细胞存活来确证漂浮细胞可以延迟再水化。结果:部分漂浮细胞更换培养瓶后,延迟水化,再次贴壁,检测代谢指标显示细胞代谢功能恢复。讨论:冻伤后部分细胞由于脱水出现休眠状态,予以合适条件后细胞可以再次复苏、水化。Objective:To verify the phenomenon of delayed rehydration of normal human fibroblasts after freezing and thawing. Methods: Samples were cooled in a rate of 1 0. 1℃/ min down to the experimental temperature of - 10℃, and they were warmed at 20℃. After being cultured for one day the cells suspending in the medium were collected and seeded. The adherent rate and mitotic index of the fibroblast were assessed. MTT test -was used to verify the phenomenon of rehydration of survived fibroblasts. Result: After freezing and thawing, the fibroblasts still had ability to adhere. MTT test showed that the cells survived. The experiment verified the phenomenon of delayed rehydration of normal human fibroblasts after freezing and thawing. Conclusion: Rehydration is a kind of protective mechanism,and when the offence of low temperature is removed, the cell might revive.

关 键 词：低温处理 成纤维细胞 复苏过程 延迟 再水化 FREEZING and thawing 细胞培养 细胞贴壁率 休眠状态 细胞复苏 细胞代谢 细胞存活 脱水 检测 合适条件 过程存在 功能恢复 分裂指数 代谢指标 噻唑蓝 

分 类 号：R[医药卫生]