Np63α在创面愈合过程中表达改变的研究  

ΔNp63α expression during normal healing of cutaneous wound

作　　者：雷永红[1] 郭永峰 付小兵[1] 盛志勇[1] 孙同柱[1] 赵京禹[1] 

机构地区：[1]解放军总医院第一附属医院全军创伤修复重点实验室,北京100037 [2]第四军医大学西京医院整形外科,西安710032

出　　处：《感染．炎症．修复》2006年第2期67-69,129,共4页Infection Inflammation Repair

基　　金：国家重点基础研究发展规划资助项目(2005CB522603);全军十一五课题(069116);国家自然科学基金资助项目(30400172)

摘　　要：目的：探讨p63基因编码的蛋白亚型△Np63α在创面愈合表皮再生中的表达及其生物学意义。方法：①在3周龄小鼠背部用直径1．6cm的环钻压切制备出一个2．0cm^2全层皮肤缺损的圆形创面,并将切除的皮肤原位缝合以覆盖创面。②将18只昆明小鼠随机分成两组,实验组12只小鼠均在背部用环钻制备圆形创面；而对照组6只小鼠不致伤。分别于1d、2d、3d、7d、14d、21d在实验组创缘和对照组小鼠背部相同部位切取全层皮肤组织标本,用抗鼠多克隆抗体P40进行抗△Np63免疫组化染色。结果：△Np63α在正常皮肤中的特征性表达模式是强阳性染色限于生发层的角质形成细胞核。伤后1～2d,表皮开始迁移,与对照组正常皮肤相比,迁移表皮舌基底层细胞△Np63α表达下调。伤后3d,△Np63α有较强的表达出现在创缘和迁移的表皮舌中,阳性染色限于基底层和其上方1～2层的角质形成细胞,而创面尚未被上皮覆盖处则没有阳性细胞。伤后5～7d,△Np63a有更强的表达出现在创缘和迁移表皮舌的基底层角质形成细胞。伤后14～21d创面愈合,△Np63α在覆盖创伤区表皮的基底层和棘层的细胞中高表达,并且其表达在表皮中长期和持续的存在。结论：△Np63α可能通过调控表皮修复中的细胞增殖和细胞迁移而参与正常皮肤的创面愈合过程。Objective:p63,a recently identified member of the p53 family,was shown to play a role in morpho- genesis.Because p63 is a marker of keratinocytes with a high proliferative potential,the expression of this protein was studied to indicate its biological significance during normal epidermal regeneration in mice.Methods:Circular and full-thickness skin measuring 2.0 cm^2 in size were first from the back and sutured back in 12 mice ,and serial biopsies of skin from the margin of the wound were obtained at various time intervals(1,2 ,3,7,14 and 21 days after the injury),and they were studied immunohistochemically with the use of a P40 monoclonal antibody against theΔNp63 variant.Control group consisted of 6 mice,from them biopsy of skin was taken at similar sites at the same time as the experimental group,and similar studies were done.Results:In the normal and injured skin,the expression of theΔNp63αprotein was restricted to the epidermal keratinocytes and hair follicle keratinocytes.On the first day of the healing process,there was a down-regulation of bothΔNp63αexpression in the area of the epi- dermal basal layer invading under the crust.Three days after injury,the induction ofΔNp63αin basal keratino- cytes could be detected,followed by a gradual increase of its expression in subsequent days.Five to seven days af- ter injury ,the expression ofΔNp63αin basal keratinoeytes was stronger than before.Several days after complete wound closure(fourteen to twenty one days after the injury),ΔNp63αwas still strongly expressed not only in the basal keratinocytes but also in the entire spinous layer,and this kind of expression pattern existed persistently. Conclusion:ΔNp63αcould be involved in the control of physiologic processes,such as cell proliferation and migra- tion,related to epidermal repair during healing of normal skin.

关 键 词：创面愈合 角质形成细胞 表皮 全层皮肤缺损 昆明小鼠 细胞迁移 对照组 基底 阳性细胞 实验组 免疫组化染色 背部 生物学意义 多克隆抗体 组织标本 圆形 原位缝合 愈合过程 细胞增殖 基因编码 

分 类 号：R[医药卫生]