冬凌草甲素对鼻咽癌HNE-1细胞株生长及其PCNA、Caspase-3表达的影响  

Effects of oridonin on growth and PCNA,Caspase-3 expression of human nasopharyngeal carcinoma HNE-1 cell lines

作　　者：向银洲[1] 余林[2] 魏莲枝[2] 贵平[1] 彭平[1] 熊志洁[1] 

机构地区：[1]宜昌市第一人民医院,湖北宜昌443000 [2]重庆医科大学附属第一医院

出　　处：《山东医药》2010年第50期20-22,共3页Shandong Medical Journal

摘　　要：目的观察冬凌草甲素对人鼻咽癌细胞株HNE-1生长及其细胞核抗原(PCNA)、Caspase-3表达的影响。方法 HNE-1中加入冬凌草甲素,MTT法观察其生长情况,FCM检测细胞周期,免疫细胞化学染色法检测PCNA,Western-blot技术检测Caspase-3。结果加入冬凌草甲素后HNE-1细胞生长受抑制,细胞被阻滞于G_0/G_1期;冬凌草甲素作用48 h后,HNE-1细胞的PCNA蛋白表达下调,Caspase-3蛋白表达增加。结论冬凌草甲素在体外可显著抑制人鼻咽癌细胞株HNE-1细胞生长并诱导其凋亡,其机制可能是下调PCNA蛋白表达,激活Caspase-3的活性。Objective To observe the effect of oridonin on growth of human nasopharyngeal carcinoma HNE-1 cell lines and its effect on proliferation cell nuclear antigen(PCNA) and Caspase-3 expression.Methods HNE-1 was cultured with oridonin.Growth of HNE-1 cells was observed by MTT assay.Cell cycle was measured by Flow cytometry(FCM.). The expression of PCNA was assessed by immunocytochemistry method.The protein expression of Caspase-3 was examined by Western-blot.Results The growth of HNE-1 cells were inhibited by or...

关 键 词：冬凌草甲素 鼻咽癌 HNE-1细胞株 细胞核抗原 半胱氨酸蛋白酶 

分 类 号：R739.63[医药卫生—肿瘤]