细丝蛋白A对药物诱导的乳腺癌细胞凋亡的影响  

Influence of filamin A on drug-induced apoptosis of breast cancer cells

作　　者：李小翠[1] 袁博 刘秀花[1] 赵玲玲[1] 王树东[3] 袁长吉[1] 

机构地区：[1]吉林大学第一医院肿瘤中心,吉林长春130021 [2]吉林大学白求恩医学院 [3]吉林大学第一医院心血管中心,吉林长春130021

出　　处：《吉林大学学报（医学版）》2011年第6期1070-1074,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅自然科学基金资助课题(201115048)

摘　　要：目的:研究细丝蛋白A(FLNa)对药物诱导的乳腺癌细胞凋亡的影响,探讨其影响细胞凋亡的具体机制。方法:不同浓度(2.5、5.0、10.0和20.0μmol.L-1)的氯化甲基汞(MMC)、三氧化二砷(AS2O3)及顺铂(DDP)分别作用MDA-MB-231/MB 231-KD(FLNa+/FLNa-)2种乳腺癌细胞,同时设空白对照组,采用MTT比色法检测各组乳腺癌细胞增殖抑制率。不同浓度(2.5、5.0、10.0和20.0μmol·L-1)MMC分别作用MDA-MB-231/MB 231-KD(FLNa+/FLNa-)2种乳腺癌细胞,同时设空白对照组,用流式细胞仪测定各组乳腺癌细胞的细胞凋亡率。结果:与空白对照组比较,2.5、5.0、10.0及20.0μmol·L-1 MMC组FLNa+和FLNa-细胞的增殖抑制率明显增加(P<0.05),与AS2O3及DDP组比较抑制率也明显增加(P<0.05);2种乳腺癌细胞之间比较,FLNa-乳腺癌细胞较FLNa+细胞抑制率明显增高(P<0.05)。流式细胞术结果显示,随着MMC剂量的增加凋亡细胞数增加,与空白对照组比较差异均有统计学意义(P<0.05),FLNa-细胞较FLNa+细胞凋亡率高,差异有统计学意义(P<0.05)。结论:FLNa的表达可以使药物诱导的乳腺癌细胞凋亡作用减弱。Objective To investigate the influence of filamin A(FLNa) on drug-induced apoptosis of breast cancer cells and explore its mechanism.Methods MDA-MB-231/MB 231-KD(FLNa+/ FLNa-) cells were treated with different doses(2.5,5.0,10.0 and 20.0 μmol·L-1)MMC,AS2O3 and DDP,meanwhile control group was set up.The inhibitory rates of proliferation of breast cancer cells were detected with MTT method.MDA-MB-231/MB231-KD(FLNa+/FLNa-)cells were treated with different doses(2.5,5.0,10.0 and 20.0 μmol·L-1)MMC,meanwhile control group was set up.The apoptotic rates of breast cancer cells were determined with flow cytometry.Results Compared with control group,the inhibitory rates of proliferation of FLNa+ and FLNa-cells in 2.5,5.0,10.0,20.0 μmol·L-1 MMC groups were significantly increased(P<0.05),and were higher than those in AS2O3 and DDP groups(P<0.05);there was significant difference between two kinds of breast cancer cells,the inhibitory rate of FLNa-cells was higher than that of FLNa+ cells(P<0.05).As the dose increasing,the number of apoptotic cells was increased,there were significant differences compared with control group(P<0.05);the inhibitory rate of FLNa-cells was higher than that of FLNa+ cells and there was significant difference(P<0.05).Conclusion The expression of FLNa can reduce the drug-induced apoptosis of breast cancer cells.

关 键 词：细丝蛋白A 细胞凋亡 乳腺肿瘤 

分 类 号：R737[医药卫生—肿瘤]