枕大池二次注血法建立大鼠蛛网膜下腔出血模型及尼莫地平的脑保护作用  

Establishment of rat subarachroid hemorrhage model by second injection of blood into cisterna and protective effect of nimodipine on brain

作　　者：吴冰[1,2] 康劲松[3] 王贺元[4] 晞欣[3] 邱海洋[3] 王伟伟[3] 罗祺[1] 

机构地区：[1]吉林大学第一医院神经外科,吉林长春130021 [2]吉林大学中日联谊医院神经外科,吉林长春130033 [3]吉林大学白求恩医学院病理生理学教研室,吉林长春130021 [4]吉林大学第一医院内分泌科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2011年第6期1153-1156,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅白求恩专项基金资助课题(200705120)

摘　　要：目的:建立一种适于研究蛛网膜下腔出血(SAH)诱发脑血管痉挛(CVS)的大鼠模型,并探讨尼莫地平的脑保护作用。方法:将Wistar大鼠分为假手术组(枕大池内注入0.3mL生理盐水)、SAH模型组(无抗凝自体血0.3mL注入枕大池)及SAH尼莫地平治疗组(简称治疗组,建模后30min及术后每日均腹腔注射尼莫地平0.2mg·kg-1)。于实验第1、7、14和21天观察大鼠神经功能障碍及进食量下降的发生率,经颅多普勒超声(TCD)检测大鼠基底动脉的最大血流速度(VmBA)。结果:与假手术组比较,SAH模型组大鼠VmBA与神经功能障碍及进食量下降(包括2～4级)的发生时程相一致,主要是第1天增加(P<0.05),第7天达到高峰(P<0.05),第14天下降(P<0.05),第21天无明显异常,且死亡率为0。与SAH模型组比较,治疗组大鼠的VmBA减慢(P<0.05),大鼠神经功能障碍及进食量下降的发生率低(P<0.05)。结论:通过枕大池二次注血法,建立了一种接近于临床、适于研究SAH诱发CVS的大鼠模型,尼莫地平可以明显地减轻SAH发生后CVS的程度。Objective To establish rat model fit to study cerebral vasospasm(CVS) induced by subarachnoid hemorrhage(SAH).Methods Wistar rats were divided into sham operation group(injected with of 0.3 mL saline into the magnum pool),SAH model group(injected with 0.3 mL no anticoagulation autologous blood into the cisterna magna),SAH nimodipine treatment group(injected introperitoneally with 0.2 mg·kg-1 nimodipine 30 min after modeling and every day after operation).The neurological deficits of rats and incidence of decreased food intake were observed and the largest rat basilar artery blood flow velocity(Vmba) was detected with transcranial Doppler(TCD) at the 1st,7th,14th and 21th after operation.Results Compared with sham operation group,the basilar artery blood flow,nerve dysfunction and decreased food intake(including 2 to 4) in SAH model group were consistent with the occurrence of schedule,mainly increased at the 1st day,peaked on day 7,decreased on day 14,normal on day 21,and the mortality rate was 0.Compared with SAH model group,the basilar artery blood flow velocity in treatment group was decreased,the incidence of nerve dysfunction and decreased food intake were decreased also.Conclusion Through the second injection of blood into cisterna magna,a rat model closing to clinic and fiting for research on CVS induced by SAH is established,and nimodipine can significantly reduce the degree of CVS after SAH.

关 键 词：二次注血法 蛛网膜下腔出血 脑血管痉挛 疾病模型 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]