人类疱疹病毒6型与口腔恶性肿瘤相关性的研究  

The Correlation between Human Herpesvirus-6 (HHV-6) and Oral Malignancy.

作　　者：杨婕[1] 朱玲[2] 王芳[3] 姚堃[3] 

机构地区：[1]南京医科大学口腔医学院2003级硕士六班,江苏南京210029 [2]江苏省口腔医院儿童牙病科 [3]南京医科大学微生物与免疫教研室

出　　处：《口腔医学研究》2005年第4期371-374,共4页Journal of Oral Science Research

基　　金：国家自然科学基金资助项目(编号:39270034)获得2001年江苏省科技进步二等奖

摘　　要：目的:研究人类疱疹病毒6型(HHV-6)的感染与口腔恶性肿瘤的相关性及其在临床诊疗中的意义。方法:运用巢式PCR技术,对40例口腔恶性肿瘤患者的血液、唾液、癌组织、正常组织及40例正常对照人群血液、唾液中人类疱疹病毒6型DNA进行检测分析。结果:恶性肿瘤组和对照组唾液中HHV-6 DNA阳性率分别为85.0%、45.0%,其差别具有统计学意义(P<0.01);两组血液中HHV-6检出阳性率分别为67.5%、22.5%,其差别亦具有统计学意义(P<0.01)。两组唾液中的阳性检出率均大于血液。癌组织阳性检出率(78.95%)显著大于正常组织(10.53%),具有统计学意义(P<0.01)。结论:进一步证实了唾液腺是HHV-6长期潜伏和增殖的部位,唾液是其主要的传播媒介,为临床简便有效诊断提供参考。HHV-6感染与口腔恶性肿瘤之间存在相关性,可能参与肿瘤发生、细胞癌变的过程。Objective： To investigate the clinical significance of human herpesvirus -6＇s（ HHV -6 ）infection and its corre lation to oral malignancy. Methods： Samples of saliva, peripheral blood mononuclear cells （ PBMCs）, cancer tissue and normal tissue collected from 40 patients with oral malignancy and 40 healthy young adults were tested for human herpesvirus -6 by nested PCR assays. Results： HHV -6 DNA positive rates were 85.0% and 45.0% in the salivas of patients and control group respectively, 67.5% and 22.5% in the PBMCs of patients and control group respectively, both of which demonstrated statistical difference. In both groups HHV -6 DNA positive rates in saliva were higher than those in PBMCs. The positive rate in cancer tissues（78.95% ） was significantly higher than that of normal tissues（ 10.53% ）. Conclusion： The result confirms that saliva gland is the latenty and proliferation position of HHV - 6 and saliva is the predominant transmission route of HHV -6. The infection of HHV -6 correlates to oral malignancy, which suggests HHV -6 may play a role in the occurrence and cell transformation of malignancy.

关 键 词：人类疱疹病毒6型 口腔恶性肿瘤巢式聚合酶链反应 

分 类 号：R739.8[医药卫生—肿瘤]