共轭亚油酸对饮食诱导肥胖大鼠脂代谢及相关基因表达的影响  

EFFECT OF CONJUGATED LINOLEIC ACID ON LIPID METABOLISM AND EXPRESSION OF RELATIVE GENES IN OBESE RAT FED WITH HIGH FAT DIET

作　　者：周晓蓉[1] 孙长颢[1] 刘荣[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院卫生毒理教研室,哈尔滨150086

出　　处：《营养学报》2005年第4期292-295,299,共5页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30271118)

摘　　要：目的:通过研究共轭亚油酸(CLA)对饮食诱导肥胖大鼠脂肪酸转移蛋白、酰基CoA合成酶基因表达的影响,探讨CLA抗糖尿病机制。方法:选雄性Wistar大鼠,随机分为对照组、高脂组、高脂+CLA组(每100g饲料含CLA分别为0.75、1.50、3.00g),每组动物10只,观察CLA对肥胖大鼠血清游离脂肪酸(FFA)、胰岛素、血糖水平的影响,并应用RT-PCR法检测脂肪酸转移蛋白(FATP)、酰基CoA合成酶(ACS)、过氧化物酶体增殖物活性受体γ(PPARγ)mRNA的表达水平。结果:高脂组大鼠血清FFA、胰岛素和血糖水平显著高于对照组,CLA可降低肥胖大鼠血清FFA、胰岛素、血糖水平,并且可增加肥胖大鼠脂肪组织FATP、ACS、PPARγmRNA的表达水平。结论:CLA可通过激活PPARγ上调FATP、ACS基因的表达,改善肥胖大鼠的胰岛素抵抗。Objective： To examine the effect of conjugated linoleic acid （CLA） on lipid metabolism and genes expression of fatty acid transport protein （FATP） and acyl-CoA synthase （ ACS ） in white adipose tissue of rats. Method： Male Wistar rats were randomly divided into control group, high-fat group and high fat ＋CLA group （0.75, 1.50, 3.00 g/100g bw）. By reverse transcription polymerse chain reaction （RT-PCR） technique the expression levels of FATE ACS and peroxisome proliferator-activated receptor- γ （PPAR γ） mRNA were detected. Results： Serum FFA, insulin and glucose levels of rats fed with high fat diet were higher than those of control group. Supplement of CLA can decrease serum FFA, insulin and glucose levels of obese rats, and increase expression of FATP, ACS and PPAR γ mRNA in adipose tissue of obese rats. Conclusion： CLA can improve insulin resistance of obese rat and increase expression of FATP, ACS mRNA, possibly acting through activating PPAR γ.

关 键 词：共轭亚油酸 脂肪酸转移蛋白 酰基辅酶A合成酶 过氧化物酶体增殖物激活受体Γ 胰岛素抵抗 

分 类 号：R589.2[医药卫生—内分泌]