经通口服液中阿魏酸含量测定方法的研究  

HPLC Determination of Ferulic Acid in Jingtong Oral Solution

作　　者：谢先吉[1] 王临润[1] 吴文娟 

机构地区：[1]浙江大学医学院附属第一医院,杭州310003 [2]浙江绍兴兰亭集团,绍兴312000

出　　处：《药物分析杂志》2005年第8期992-993,共2页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:测定经通口服液中阿魏酸的含量。方法:用反相高效液相色谱法,ODS C_(18)色谱柱(4.6mm×250mm,5μm),流动相为乙腈-1%醋酸水溶液(15:85),检测波长315nm,流速1.0 mL·min^(-1),柱温为30℃。结果:经通口服液中阿魏酸与其他成分良好分离,阿魏酸的保留时间15.30 min,线性范围16.24～81.20 ng,r=0.9999,平均回收率101.9%,RSD 为1.4%。结论:本法灵敏度高,操作简便,结果准确,可用于经通口服液中阿魏酸的含量测定。Objective： To determine the concentration of ferulic acid in Jingtong oral solution with HPLC. Method： Determined by HPLC using ODS C18 （4.6 mm × 250 mm, 5μm） chromatographic column, acetonitrile - 1% acetic acid solution（ 15： 85）as mobile phase. The wavelength for detection was 315 nm, the flow rate was 1.0 mL·min^-1 and the temperature was set at 30℃. Results：The peak of ferulic acid appears on about 15.30 minutes, the range of calibration curve was 16. 24 - 81.20 ng, r = 0. 9999, the method recovery of ferulic acid was 101.9% （RSD = 1.4% ）. Conclusion ：This method is sensitive, quick and accurate for measurement of ferulic acid concentration in Jingtong oral solution.

关 键 词：阿魏酸 经通口服液 高效液相色谱法 含量测定方法 口服液 反相高效液相色谱法 C18色谱柱 平均回收率 检测波长 保留时间 

分 类 号：R927.2[医药卫生—药学] R286.0