宫颈癌细胞TRAIL死亡受体和诱骗受体表达的研究  

Expressions of death receptors and decoy receptors of TRAIL in cervical carcinoma cells

作　　者：朱琳[1] 李慧荣[1] 吴建[1] 马秀明[1] 刘琪[1] 纪红玉[1] 

机构地区：[1]山东大学第二医院妇产科,济南250033

出　　处：《现代妇产科进展》2005年第4期277-280,共4页Progress in Obstetrics and Gynecology

摘　　要：目的:检测宫颈癌细胞HeLa表面TRAIL死亡受体(DR4、DR5)及诱骗受体(DcR1、DcR2)的表达差异,研究HeLa细胞系对TRAIL蛋白诱导的凋亡敏感程度。方法:应用MTT法检测TRAIL蛋白对人宫颈癌细胞HeLa的诱导凋亡率,分析宫颈癌细胞对TRAIL蛋白的敏感程度;应用逆转录聚合酶链反应技术(RTPCR)、流式细胞术,检测人宫颈癌细胞HeLa表面TRAIL受体DR4、DR5、DcR1、DcR2mRNA和蛋白的表达,分析宫颈癌细胞表面死亡受体DR4、DR5与诱骗受体DcR1、DcR2表达的相对程度。结果:HeLa细胞对TRAIL诱导的细胞凋亡反应有较高的敏感性,在较低浓度(100ng/ml)TRAIL的作用下,即有接近50%的细胞杀伤率。HeLa细胞表面DR4、DR5受体mRNA的表达明显高于DcR1、DcR2受体,差异有显著性(P<0.01),DR4、DR5受体之间及DcR1、DcR2受体之间表达差异无显著性(P>0.01)。HeLa细胞表面DcR1,DcR2受体蛋白的表达率(17.7%、5.3%)明显较DR4,DR5受体表达率(99.9%、97.8%)低(P<0.01)。结论:宫颈癌细胞HeLa对TRAIL诱导的细胞凋亡反应有较高的敏感性,而且DcR1、DcR2在宫颈癌细胞表面的表达程度相对低于DR4、DR5,将TRAIL用于治疗宫颈癌具有潜在的应用前景。Objective：To study the expression of death receptors （DR4,DR5）and decoy receptors （DcR1, DcR2） of TRAIL in cervical carcinoma cell line Hela,and analyze the sensitivityof cervical carcinoma cells to TRAIL.Methods： The rate of TRAIL-induced apoptosis in cervical carcinoma cell is examined by MTT method, then the sensitivity of HeLa towards TRAIL was analyzed.The expressions of DR4, DR5 and DcR1, DcR2 in HeLa were detected by reverse transcription-polymerase chain reaction （RT-PCR） and flow cytometry methods （FCM）,then the comparative difference of death receptors and decoy receptors in HeLa was analyzed.Results： At the lower concentration （ 100ng/ml ） of TRAIL, about 50% of cells were killed.The mRNA expressions of death receptors in HeLa were significantly higher than those of decoy receptors （ P 〈0.01 ）. Expressions of receptor protein in HeLa were DcR1 17.7% ,DcR2 5.3%, DR4 99.9%, DR5 97.8% respectively.Conclusion：The human cervical carcinoma cell line has a high sensitivity towards TRAIL and the expressions of DcR1 and DcR2 in HeLa were much lower than those of DR4 and DR5 ,which might be a clue for application of TRAIL in molecular therapy of human cervical carcinoma.

关 键 词：宫颈肿瘤 肿瘤细胞 培养的 肿瘤坏死因子相关凋亡诱导配体 死亡受体 诱骗受体 

分 类 号：R737.33[医药卫生—肿瘤]