白皮松不定芽增殖和生长研究  

Study on propagation and growth of adventitious buds of Pinus bangeana

作　　者：李林[1] 唐德瑞[2] 张海忠[3] 李科友[2] 

机构地区：[1]中国科学院华南植物园鼎湖山树木园,广东肇庆526070 [2]西北农林科技大学林学院,陕西杨凌712100 [3]福建农林大学林学院,福建福州350002

出　　处：《西北农林科技大学学报（自然科学版）》2005年第8期107-110,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家林业局948引进项目"美国黄松优良种源及快繁技术引进"(98-4-05);国家林业局黄土高原林木培育实验室项目"美国黄松组织培养及快繁技术研究"(K02-06)

摘　　要：研究了基本培养基、激素、活性炭和GA3在白皮松不定芽增殖和生长培养过程中的作用。结果表明,采用MS培养基白皮松不定芽的增殖系数均超过5。培养基中加入0.05mg/L分裂素时不定芽的增殖系数最高达到6.7。培养基中添加活性炭对不定芽增殖的影响不大,但对不定芽的生长,特别是不定芽的伸长具有明显效果,在3g/L活性炭的培养基中,高度大于2cm的嫩梢达到42.3%,大于4cm的嫩梢达到28.4%。在基本培养基中添加GA3有助于白皮松不定芽的伸长,但GA3质量浓度过高又会抑制其伸长。The thesis studied the use of mediums ,hormone,activated charcoal and GA3 in adventitious buds of Pinus bangeana proliferation and growth. The results showed MS was the optimal mediums ,the adventitious buds proliferation rate was above 5. Added 0. 05 mg/L NAA, without 6-BA, the adventitious buds proliferation rate was 6.7. Added activated charcoal in mediums was no use to adventitious buds proliferation. But it was helpful for adventitious buds growth ,especially for buds elongation. Added 3 g/L activated charcoal in MS mediums, the rates of height≥2 cm shoot reached 42. 3%, ≥4 cm shoot reached 28.4%. GA3 was also helpful for buds elongation, while high concentration was restraining the buds growth.

关 键 词：白皮松 不定芽 活性炭 GA3 

分 类 号：S791.243.05[农业科学—林木遗传育种]