偏凸山羊草的抗条锈病新基因YrG775的AFLP标记  

Tagging a novel yellow rust gene YrG775 derived from Aegilops ventricosa with AFLP marker

作　　者：张超[1] 陆和平[2] 葛昌斌[1] 徐如宏[1] 张庆勤[1] 

机构地区：[1]贵州大学小麦作研究中心,贵州贵阳550025 [2]西北农林科技大学植保学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2005年第B08期268-268,共1页Journal of Northwest A&F University(Natural Science Edition)

基　　金：贵州省小麦"十五"攻关项目(15001A5-2;15001A-9)

摘　　要：对小麦抗病种质贵农775与西农97148的F2后代人工接种CY32条锈病菌,对其进行了抗性鉴定,通过卡方检测抗感病单株分离比例,确定贵农775携带有2对重叠抗条锈病基因。从128个AFLP引物组合中,筛选到与其中1个抗病基因YrG775共分离的多态性标记M8P15(1 200 bp),该标记仅能在原始亲本偏凸山羊草中检测到。由于已知来源于偏凸山羊草的Yr17苗期不抗CY32条锈病菌,所以根据抗性鉴定和分子生物学试验结果,推断YrG775很可能是1个来自偏凸山羊草,并与已知抗条锈病基因都不同的新基因。In this study,the F2 populations crossing by Xinong 97148×Guinong 775 was inoculated with the pathology strains CY32 to test their resistance. The Chi-square tests results showed the yellow rust resistance of Guinong 775 was controlled by two duplicate genes. Among 128 AFLP primer combinations ,the primer combination M8P 15 had a polymorphic band about 1 200 bp linked to the YrG775 （temporarily designed） ,one of the two duplicate genes. F2 segregations analysis confirmed the band co-segregated with this gene and only Aegilops ventricosa ,one of the original parents of Guinong 775 had this polymorphic band. Since the Yr17,the only yellow rust resistance gene known to be derived from Aegilops ventricosa,had no resistance to the pathology strains CY32,the gene YrG775 identified in this paper might be a novel yellow rust resistance gene.

关 键 词：AFLP 偏凸山羊草 抗条锈病基因 YrG775 小麦 

分 类 号：S512.103.4[农业科学—作物学]