血管性痴呆小鼠海马NMDA受体mRNA表达特征及喜得镇的影响  

The effect of hydergine on N-methyl-D-aspartate receptor in neuron of hippocampus in mice with vascular dementia in situ hybridization

作　　者：梁翠萍[1] 樊敬峰[1] 吕佩源[1] 王伟斌[2] 尹昱[3] 

机构地区：[1]河北省人民医院神经内科,石家庄050051 [2]山东省济宁市第一人民医院神经内科 [3]河北省人民医院康复科,石家庄050051

出　　处：《卒中与神经疾病》2005年第4期212-214,共3页Stroke and Nervous Diseases

基　　金：河北省自然科学基金资助项目(301415)

摘　　要：目的观察喜得镇对血管性痴呆(vasculardementia,VD)小鼠海马神经元N甲基D天门冬氨酸(NmethylDaspartate,NMDA)受体原位杂交变化的影响,并探讨NMDA受体原位杂交变化特征及喜得镇对其变化的影响。方法双侧颈总动脉结扎,反复缺血再灌注法制备模型,药物组用喜得镇溶液灌胃,跳台试验和水迷宫试验观察其行为学改变,采用原位杂交技术观察小鼠海马神经元NMDA受体的表达变化。结果药物组小鼠学习、记忆成绩优于模型组(P<0.01),其海马N甲基D天门冬氨酸受体表达也明显增高(P<0.01)。结论喜得镇能改善血管性痴呆小鼠模型的学习、记忆功能,这可能与N甲基D天门冬氨酸受体mRNA水平正常化有关。Objective To observe the effect of hydergine on N-methyl-Daspartate receptor mRNA in neuron of hippocampus in mice with vascular dementia. Methods The mice were subjected for reperfusion repeatedly on bilateral common carotid arteries by knots to establish the VD models. The behavioral abnormalities were investigated by step-down test and water maze test. Hydergine was given to the mice in medication group and N-methyl-D-aspartate receptor mRNA in neuron of hippocampus were observed in situ hybridization technique. Results Hydergine improved the grades of learning and memory in VD mice. The changes of N-methyl- D-aspartate receptor mRNA in neuron of hippocampus were ameliorated by hydergine. Conclusions Hydergine can improve dysfunction of learning and memory in VD, which suggest that hydergine may restore expression of N-methyl-Daspartate receptor mRNA.

关 键 词：血管性痴呆 N-甲基D-天门冬氨酸 喜得镇 原位杂交 

分 类 号：R749.13[医药卫生—神经病学与精神病学]