大肠杆菌O_(157):H_7 rfbE基因和fliC基因的克隆与序列分析  

Cloning and Sequencing of rfbE gene and fliC gene in E.coli O_(157):H_7

作　　者：朱文冠[1] 杨德胜 郭霄峰[1] 

机构地区：[1]华南农业大学兽医微生物教研室,广州510642 [2]广东省东莞市兽医防疫检疫站,广东省东莞523010

出　　处：《中国动物检疫》2005年第9期22-24,共3页China Animal Health Inspection

基　　金：本研究获广东省攻关项目的资助;项目号为:2002B21406。

摘　　要：根据已发表的E.coliO_(157):H_7EDL933株的rfbE基因和fliC基因,设计了两对引物,分别对两个O_(157):H_7菌株的rfbE基因和一个菌株的fliC基因进行PCR,并将PCR产物克隆于pMD18-T载体质粒。测序结果表明,获得到了与理论相符的582bprfbE基因片段和802bpfliC基因片段。一株菌的rfbE基因存在无意义突变(两个)。而另一株菌fliC基因有一个有意义突变(aac→gac)To clone and analysize rfbE and fliC genes of E.coli O157：H7 , two primers were designed according to the sequence of rfbE gene and fliC gene of E.coli O157：H7 EDL933 strain.The part fragment of rfbE gene of two E.coli O157：H7 ,882364 and 021210 strain, were amplified by polymerase chain reaction （PCR） respectively. Both PCR products were cloned into pMD-18 vector and identified by BamH I ,HindlII and EcoRI and two positive recombinants containing rfbE gene were selected and sequenced.With the same method another positive recombinant containing fliC gene from 021210 strain was analysized. The result suggested homology of the rfbE gene between 882364 and 021210 strain was 99% for nucleotide sequence . While the homology of the fliC gene compared with GENBANK sequence （AF228487）was 99%.

关 键 词：E.COLI O157:H7 rtbE基因 fliC基因 克隆 大肠杆菌O157:H7 FLIC E基因 序列分析 O157:H7 

分 类 号：Q939.121[生物学—微生物学] TP391.41[自动化与计算机技术—计算机应用技术]