膨胀床离子交换吸附分离纳豆激酶  

Purification of nattokinase by ion exchange adsorption in expanded bed

作　　者：胡洪波[1] 张雪洪[1] 梅乐和[2] 姚善泾[2] 朱自强[2] 

机构地区：[1]上海交通大学生命科学技术学院,上海200030 [2]浙江大学化学工程与生物化工系,浙江杭州310027

出　　处：《化学工程》2005年第4期1-4,共4页Chemical Engineering(China)

摘　　要：对膨胀床分离纳豆激酶过程的各个阶段进行了考察。在填充床上的探索性实验表明,吸附时最佳的pH为6.0,电导率应低于6.2mS/cm。在膨胀床上样吸附阶段,考察了保持流速不变和保持床层膨胀率不变2种操作方式,结果表明采用控制膨胀率不变的方法更适合纳豆激酶这种料液的粘度和平衡缓冲液的粘度相差不大的情况;在冲洗阶段,通过考察颗粒冲洗的效率确定采用平衡缓冲液冲洗;洗脱阶段采用填充床模式洗脱。与传统方法相比,用膨胀床对纳豆激酶进行分离和纯化,操作步骤从6步减少为2步,操作时间缩短了8—10h,回收率提高了约50%。The use of an expanded bed for purification of the nattokinase from cell-contained fermentation broth was investigated. Method-scouting experiments, carried out in packed beds, show that the optimal pH for adsorption is 6.0 and conductivity of the feed-stock shall be lower than 6.2 mS/cm. In adsorption stage of expanded bed, two modes of feed-stock application, performed with constant bed expansion and constant flow-rate, were studied. The result shows that maintaining a constant degree of expansion during the adsorption stage is more suitable for nattokinase purification because the viscosity of the feed-stock is similar to that of equilibration buffer solution. In washing stage, the particulate removal efficiency was studied and the washing was performed with the start buffer solution. The nattokinase was eluted by packed-bed mode. Compared with the conventional method, the expanded bed method for nattokinase purification can decrease the operating steps from 6 to 2 and save operating time about 8-10 h. Moreover, the activity recovery is increased by 50%.

关 键 词：吸附 膨胀床 纳豆激酶 分离纯化 

分 类 号：TQ464.8[化学工程—制药化工]