TRAIL和TRAILR在肝癌中表达及对肝癌的治疗作用  

Expression of TRAIL and TRAILR in human hepatocellular carcinoma and its therapeutic significance

作　　者：肖震宇[1] 陈孝平[1] 何松青[1] 黄志勇[1] 李莉[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院普外科,武汉430030 [2]华中科技大学同济医学院免疫学教研室

出　　处：《中华普通外科杂志》2005年第8期500-503,共4页Chinese Journal of General Surgery

基　　金：2001-2003年卫生部临床学科重点资助项目[(2001)321号]

摘　　要：目的探讨肿瘤坏死因子相关凋亡诱导配体(tumornecrosisfactorrelatedapoptosis-inducingligand,TRAIL)与其受体(TRAILR)在肝细胞肝癌中的表达及TRAIL联合应用化疗药对肝癌细胞的杀伤作用。方法采用免疫组化和原位杂交检测100例肝癌及癌旁组织中TRAIL及TRAILR的表达。用MTT法检测TRAIL与化疗药联合应用对肝癌细胞的杀伤作用。结果TRAIL在正常肝组织中无表达,在癌旁组织中的表达明显高于癌组织;肝癌组织中死亡受体(DR5、DR4)的表达量显著强于正常肝组织,诱捕受体为低表达,与正常肝组织相比,两者差异显著(χ2=4·68,P<0·05)。Ⅲ/Ⅳ期肿瘤死亡受体的表达显著低于Ⅰ/Ⅱ期(χ2=6·17,P<0·05)。联合使用亚细胞毒性剂量的化疗药大幅度提高了TRAIL的细胞毒活性。结论TRAIL与化疗药物联合运用具有协同杀伤肝癌细胞的作用。Objective To evaluate the expression ot tumor necrosis tactor related apoptosis- inducing ligand （TRAIL） and its receptor（TRAILR） in human hepatocellular carcinoma （HCC） , and its therapeutic potential for human HCC. Methods Expressions of TRAIL and TRAILR were determined in and adjacent to carcinoma tissues, and normal liver tissues. The cytotoxic effects of TRAIL in combination with chemotherapeutic agents on HCC cell lines were detected. Results Expression of TRAIL in tumor adjacent tissue was higher than in cancer tissue. There were high expression of DR and low expression of DcR in HCC tissue in contrast to normal hepatic tissue（X^2 =4. 68 ,P 〈0. 05）. The expression of DR in stage Ⅲ - Ⅳ was significantly lower than that in stage Ⅰ - Ⅱ （X^2 = 6. 17, P 〈 0. 05 ）. Chemotherapeutic agents enhance TRAIL induced cytotoxic function. Conclusion TRAIL in combination with chemotherapeutic agents shows strong cytotoxic effect on HCC cell lines.

关 键 词：TRAIL TRAILR 肝癌 基因表达 肿瘤坏死因子相关凋亡诱导配体 细胞毒性 生物疗法 

分 类 号：R735.7[医药卫生—肿瘤]