免疫初乳中IgG的分离与纯化  

Isolation and Purification of IgG from Immune Colostrums

作　　者：范丽娟[1] 潘道东[2] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210097 [2]南京师范大学金陵女子学院食品科学与营养系,江苏南京210097

出　　处：《食品科学》2005年第8期146-149,共4页Food Science

基　　金：国家十五奶业重大科技专项(2002BA518A12);江苏省十五攻关项目(BE2002302);江苏省自然科学基金(BK2005137)

摘　　要：本实验采用硫酸铵一次性盐析、DEAE-SepharoseFF离子交换层析和SephacrylS-200凝胶过滤层析法,分离纯化免疫初乳中的IgG,并采用SDS-PAGE电泳、单向火箭免疫电泳及试管凝集法对IgG分离物进行了定性定量检测,结果表明所得IgG纯度高,活性大。IgG precipitated by ammonium sulfate from Immune colostrum was isolated and purified by DEAE-Sepharose FF Ion exchange chromatography and Sephacryl S-200 Gel filtration chromatography. IgG was validated by SDS-PAGE electrophoresis, one-dimension rocket immunoelectrophoresis and the methods of tube agglutination. The results demonstrated that based on high activity, the purity of IgG was significant increased.

关 键 词：免疫初乳 IGG 分离纯化 

分 类 号：TS252.59[轻工技术与工程—农产品加工及贮藏工程]