藻蓝蛋白对离体蟾蜍心脏正性肌力作用研究  

A Study on the Positive Inotropic Action of Phycocyanin on Isolated Toad Heart

作　　者：辛晓林[1] 杨立红[1] 黄清荣[1] 冯芹[1] 

机构地区：[1]烟台师范学院生物系,山东烟台264025

出　　处：《食品科学》2005年第8期384-386,共3页Food Science

摘　　要：目的:研究藻蓝蛋白对心肌收缩活动的影响,并探讨其作用机理。方法:采用斯氏离体蛙心灌流方法,观察藻蓝蛋白对心肌收缩力和心率的影响。结果:三个浓度(1.2、4.5、8.4μg/ml)的藻蓝蛋白均能增强离体蛙心心肌收缩力(p<0.01),但未见其对心率的影响。心得安、异搏定均能拮抗藻蓝蛋白(8.4μg/m)的效应。结论:藻蓝蛋白具有正性肌力作用,其机制与兴奋心肌细胞膜上β受体及激活细胞膜上Ca2+通道有关。Objective： To study the effect of phycocyanin on myocardial contraction action of isolated toad heart and the mechanism involved in this action. Methods： Isolated toad heart model produced by Straub＇method was used to observe the change of myocardial contraction force and cardiac rate after phycocyanin were used. Results： At three concentration （1.2, 4.5, 8.4μg/ml）the myocardial contraction force increased extremely strikingly （p〈0.01）,But didn＇t show the effect on toad cardiac rate. Propranolol and verapamil antagonized the effectof phycocyanin （8.4μg/ml）. Conclusions： The main effects of phycocyanin were that it haspositive inotropic action, the mechanism of the action might be related to the excitation of β -receptor and calcium channel.

关 键 词：藻蓝蛋白 心脏 正性肌力 Β受体 CA^2+通道 

分 类 号：S555.6[农业科学—作物学]