釉基质蛋白对大鼠外胚间充质细胞黏附、伸展影响的体外研究  

Influence of EMPs & EMD on the attachment and spreading of ectomesenchymal stem cells in vitro

作　　者：柴枫[1] 金岩[1] 赵铱民[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2005年第8期424-427,共4页Chinese Journal of Conservative Dentistry

基　　金：第四军医大学创新工程重点课题(CX02F002)

摘　　要：目的:了解体外研究环境中,两种不同形式釉基质蛋白(粗提型EMPs和纯化型EMD)对外胚间充质细胞黏附、伸展行为的影响力。方法:酶消化法培养SD大鼠颌突外胚间充质细胞,采用固相结合分析方法,观察外胚间充质细胞在不同浓度釉基质蛋白(50、100、150及200μg/mL等浓度的EMPs和EMD)包被的培养孔表面黏附、伸展情况,依次命名为ED1、ED2、ED3、ED4及EP1、EP2、EP3、EP4组。结果:①细胞黏附性实验中,仅ED2和ED3组结果高于空白对照组(P<0.01)。②细胞伸展实验中,EP1、EP2和EP3组结果高于空白对照组(P<0.01)。结论:EMD对外胚间充质细胞伸展无明显促进作用,但可显著促进其黏附贴壁;EMPs对外胚间充质细胞黏附无影响,但可促进其伸展。AIM：To compare and determine the ability of enamel matrix proteins （EMPs ＆ EMD） to influence specific properties of attachment and spreading of rat ectomesenchymal stem cells in vitro. METHODS：Cell culture technique and solid - phase hireling assay were used to measure the amount of attached cells and the ratio of spreading cells on the EMPs - or EMD - eoated surface of culture wall, according to the concentration （50,100,150 and 200μg/mL） of protein grouped as EDI , ED2, ED3,ED4及EP1 , EP2, EP3, EP4. RESULTS ：①The amount of attached cells in ED2 and ED3 group was higher than that of the negative control （ P 〈0.01 ） ;②The ratio of spread cells in EP1 ,EP2 and EP3 group was higher than that of the negative control （P 〈 0.01 ）. CONCLUSION：There are little difference of bioeffect between EMD and EMPs,i. e. EMD can increase the attachment of ectomesenchymal stem cells, EMPs can enhance cell spreading.

关 键 词：釉基质蛋白 外胚间充质细胞 牙周组织再生 黏附 

分 类 号：R780.2[医药卫生—口腔医学]