重组人生长激素对体外培养成骨细胞的影响  

Effect of recombinant human growth hormone in cultivated osteoblasts of rats in vitro

作　　者：石勇[1] 李晓红[1] 郭三萍[1] 宋玮[2] 

机构地区：[1]西安交通大学口腔医院,710004 [2]第四军医大学口腔医学院,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2005年第8期443-446,共4页Chinese Journal of Conservative Dentistry

基　　金：陕西省科技计划项目(2003k10-G78)

摘　　要：目的:观察重组人生长激素对体外成骨细胞直接、短期的影响。方法:将不同浓度的重组人生长激素(recombinanthumangrowthhormone,RHGH)加入大鼠成骨细胞培养体系,观察不同作用时间点对大鼠成骨细胞的增殖、碱性磷酸酶活性及细胞周期的影响。结果:RHGH对成骨细胞增殖、碱性磷酸酶活性均有影响,它可促进成骨细胞增殖,提高其碱性磷酸酶活性,且可促使细胞周期中S期、G2/M期细胞百分比增加,其作用强度随浓度增加有增强趋势。结论:RHGH对成骨细胞的合成代谢有直接影响,对成骨细胞的增殖、碱性磷酸酶活性影响远强于雌激素。AIM：To investigate the direct, short term effects of recombinant human growth hormone （ RHGH ） on rats osteoblasts cultured in vitro. METHODS ：Osteoblastic cells from rats were incubated by adding rh- gh at different doses in different time,and then cell proliferation,the activity of alkaline phosphatase （ALP） and cell cycle were observed. RESULTS：RHGH stimulate proliferation and ALP activity of osteoblasts. At the same time, S phase and G2/M phase percentage was significantly higher as compared with the control. The effects tended to get stronger as the concentrations increased. CONCLUSION：The results obstained in this study suggest that rh -gh exerts direct anabolic effects on osteoblasts. The effects of rh - gh were higher than the estrogen.

关 键 词：成骨细胞 重组人生长激素 细胞增殖 

分 类 号：R780.2[医药卫生—口腔医学]