pp60c-src在凝血酶诱血管平滑肌细胞增生中的作用  

The Role of pp60 c-src in Thrombin-induced VSMCs Proliferation

作　　者：方正旭[1] 王伶[1] 刘东[1] 程晓曙[1] 

机构地区：[1]江西医学院第二附属医院心内科,江西南昌330006

出　　处：《江西医学院学报》2005年第4期12-15,共4页Acta Academiae Medicinae Jiangxi

摘　　要：目的探讨凝血酶参与血管平滑肌细胞(VSMCs)异常增生的时相关系,及pp60c-src在其增生信号传递过程中的作用。方法本实验采用培养大鼠胸主动脉3-10代的VSMCs的单细胞悬液用于实验。用MTS法测凝血酶促VSMCs细胞增殖率。用免疫沉淀测定pp60c-src的活性。结果①0.5(umol/ml)和1.0(umol/ml)两种浓度的凝血酶促VSMCs增殖相对于对照组,在各时间段均有明显促增殖作用,且二者作用程度相似,呈双峰样改变,增殖峰在1h(P<0.05)和24h(P<0.05)。②凝血酶刺激组pp60c-src活性在1h(P<0.05)及12h(P<0.05)均明显上升。相对凝血酶组,PP1有效的降低了凝血酶诱导VSMCs1h峰值(P<0.05)及24h的峰值(P<0.05)。结论①凝血酶促VSMCs的增殖在时间上呈双峰样改变,提示凝血酶不仅有早期快速促VSMCs的增殖作用,而且有后期促VSMCs的增殖作用。②由于pp60c-src活性第二高峰(12h)在凝血酶促VSMCs第二增殖峰值(24h)之前,提示pp60c-src活性后期的增加参与了凝血酶促VSMCs后期增殖。Objective To investigate the effect of thrombin on Vascular Smooth Muscle Cells （VSMCs） proliferation and the role of pp60 c-src activity on thrombin-induced VSMCs proliferation. Methods Growth-arrested VSMCs （3-10 generation） were used in the experiment. The activity of pp60 c-src in cells was assayed byimmunoprecipitation. Cell proliferation was quantified by MTS method. Results：①Either thrombin group （0.5 or 1.0 umol /ml） presents similar cell proliferation curves with two peaks occurring at 1 h（P〈0.05） and at 24 h（P〈0.05）. ②Compared with 0h, the activity of pp60c-src in thrombin group increased significantly at 1 h（P〈0.05）and at 12 h（P〈0. 05）. Compared with thrombin group, PP1 decreased the thrombin-induced cells proliferations at 1 h（P〈0.05） and at 24 h（ P〈0. 05）. Conclusion ①Cellproliferation induced by thrombin had two peaks at 1 hr and 24 hr. This indicated that induction of thrombin may not be only quickly and transient but also torpid and late. ②The increase of pp60c-src activity occurs before cell proliferation, which suggests that pp60c-src is involved in signal transmission pathway of thrombin—induced cell proliferation.

关 键 词：凝血酶 血管平滑肌细胞 pp60 c-src活性 增殖 

分 类 号：R-322[医药卫生]