血小板膜糖蛋白受体ⅠbαHPA-2基因多态性与缺血性脑血管病相关性的研究  

Association between the Polymorphisms of Platelet Membrane GlycoproteinⅠbαHPA-2 and Ischemic Cerebrovascular Disease

作　　者：刘爱华[1] 贾建平[1] 

机构地区：[1]首都医科大学宣武医院神经内科

出　　处：《首都医科大学学报》2005年第4期418-421,共4页Journal of Capital Medical University

摘　　要：目的研究膜糖蛋白(GP)ⅠbαHPA-2基因多态性与缺血性脑血管病的相关性。方法采用聚合酶链反应对正常对照组228例和缺血性脑血管病组232例患者进行研究,对所得结果进行统计学处理,进一步分组讨论,得出GPⅠbαHPA-2基因多态性与缺血性脑血管病的相关性。结果脑血管病组HPA-2基因M基因型为20.7%(T/M17.2%、MM3.5%),对照组为7.0%(T/M7.0%,MM0),经卡方检验,χ2=17.946,df=1,P=0.0002,差异有统计学意义。HPA-2基因M基因型对照组为7.0%,栓塞组为9.8%,腔隙性脑梗死组为17.5%,大面积梗死组38.2%。组间比较,对照组与栓塞组M基因型基因频率比较P=0.495,差异无统计学意义,对照组与腔隙性脑梗死组P=0.002,差异有统计学意义,对照组与大面积梗死组P=0.0001,差异有统计学意义。将GPⅠbα基因HPA-2作为应变量,脑血管病的其他危险因素作为协变量,通过多元逐步回归方法,得出HPA-2基因多态性是缺血性脑血管病独立的危险因素之一。结论血小板膜糖蛋白受体ⅠbαHPA-2基因多态性与缺血性脑血管病明显相关。Objective To research the association between the polymorphisms of platelet glycoprotein receptor Ⅰ bα and ischemic cerebrovascular disease. Methods A hospital based case-control study was conducted of 232 cases in cerebrovascular diseases group confirmed by brain CT or MRI and 228 cases without clinical evidence of CVD in control group. By Polymerase chain reaction （PCR） amplification of genomic DNA, to evaluate the interaction between the GP Ⅰ bα genotype and other variables in relation to the prevalence of CVD. Results 591 bp PCR product of HPA-2 was digested with restriction enzyme which recognizes homozygous MM,TT and heterozygous T/M. Genotypes with 145M（T/M, MM） were 20.7 % in CVD group, compared with that of the control group（7.0% ）. Statistics signs showed significant differences（ χ^2=17. 946, P= 0. 000 2）. Genotypes with 145M（T/M,MM） were 9.8% in cerebral embolism group, 17.5% in lacunar infarction group, 38.2% in large blood vessel occlusion group. When separate analysis were performed, statistics signs showed no significant differences between cerebral embolism group and control group（ χ^2 0. 465, P = 0. 495）. Contrast, statistics signs showed significant differences between lacunar infarction group, large blood vessel occlusion group and control group（ χ^2 =9. 236, P= 0. 002; χ^2 = 37.87, P = 0.000 1）. We put the glycoprotein Ⅰ bαHPA-2 as the dependent variable, other cerebrovascular disease risks as independent variable. By logistic regression analysis, to remove an influence of these factors. Conclusion The glycoprotein Ⅰ bα polymorphisms is one of the independent risks of ischemic cerebrovascular diseases.

关 键 词：血小板 膜糖蛋白受体 基因多态性 缺血性脑血管病 

分 类 号：R743.3[医药卫生—神经病学与精神病学]