Tenuigenin对转染APP695cDNA的SH-SY5Y细胞β-淀粉样蛋白生成的抑制作用及机制  

Inhibitory Effect of Tenuigenin on Beta Amyloid Protein Production of SH-SY5Y Cells Transfected with APP695 cDNA

作　　者：贾竑晓[1] 马辛[1] 姜勇[2] 阮燕[3] 张继志[1] 涂鹏飞[2] 张岱[3] 

机构地区：[1]首都医科大学附属北京安定医院 [2]北京大学药学院 [3]北京大学精神卫生研究所

出　　处：《首都医科大学学报》2005年第4期428-431,共4页Journal of Capital Medical University

基　　金：国家自然科学科学基金(39870276;39840010);国家重大基础项目研究基金(G1999064007);北京市中医药管理局科研基金(JJ99-26)资助项目

摘　　要：目的用转染APP695cDNA的SH-SY5Y细胞模型,研究tenuigenin对β-淀粉样蛋白(Aβ)生成的抑制作用及其作用机制。方法应用免疫沉淀和蛋白印迹法(Westernblot)检测Aβ含量,应用MTT和LDH法测定tenuigenin的细胞毒性。结果Tenuigenin降低SH-SY5YAPP695细胞Aβ分泌水平;无细胞增生和毒性作用;能抑制SH-SY5YAPP695细胞APPC99的生成,但对SH-SY5YSPA4CT细胞APPC99生成无抑制作用,亦不影响该细胞株Aβ的分泌。结论Tenuigenin可能通过抑制β-分泌酶对APP的水解,降低SH-SY5YAPP695细胞Aβ水平。Tenuigenin这一针对阿尔茨海默病(Alzheimerdisease,AD)上游病理环节的药效,显示了其在AD治疗方面重要的开发和临床应用的价值,值得进一步研究。Objective To investigate the inhibitory effect of tenuigenin on beta amyloid protein（Aβ） which caused Alzheimer＇s Disease（AD）. Methods Western blotting was used to test the Aβ and C99 levels of cells; MTT and LDH was used to test the neurotoxicity of tenuigenin to the cells. Results Tenuigenin can inhibit the production of beta amyloid protein of SH-SY5Y APP695 cells, didn＇t have neurotoxicity to cells; it can decrease Aβ and C99 levels of SH-SY5Y APP695 cells, but it didn＇t decrease Aβ and C99 levels of SH-SYSY SPA4CT cells. Conclusion Tenuigenin can inhibit the production of beta amylold protein of SH-SYSY APP695 cells, it＇s mechanism may come through affecting the β-secretase proteolytic activities.

关 键 词：阿尔茨海默病 Β-淀粉样蛋白 C99 免疫沉淀Western印迹法 

分 类 号：R749.06[医药卫生—神经病学与精神病学]