等鞭金藻生长和脂肪酸组成随Fe^(3+)浓度变化的研究  

Effect of ferric concentration on the growth and fatty acid composition of Isochrysis galbana

作　　者：蒋汉明[1] 翟静[1] 张媛英[1] 顾洪雁[1] 高坤山[2] 

机构地区：[1]泰山医学院天外村校区生物化学教研室,山东省泰安市271000 [2]汕头大学海洋生物研究所,广东省汕头市515063

出　　处：《中国油脂》2005年第8期57-59,共3页China Oils and Fats

基　　金：国家自然科学基金资助项目(NO.30070582)

摘　　要：研究了一种重要的海洋微量元素铁对等鞭金藻(Isochrysis galbana)生长和脂肪酸组成的影响。研究结果表明,等鞭金藻在天然海水中(铁浓度为0.5μmol/L)生长缓慢,但当培养基中Fe3+ 浓度超过12.5 μmol/L时,等鞭金藻生长差异不显著。C18:2(n-6)和C18:3(n-6)占总脂肪酸的比例随着Fe3+浓度增加而下降,但C22:6(n-6)(DHA)随着Fe3+浓度增加而上升。Fe3+浓度在12.5-120.5 μnol/L,多不饱和脂肪酸(PUFA)占总脂肪酸的比例显著下降(P<0.05)。DHA和PUFA含量(W/ W)随着Fe3+浓度的增加先上升后下降.二者达到最大值时Fe3+浓度分别为60.5和24.5 μmol/L。The growth and variation of fatty acids composition in Isochrysis galbana with different Fe^3＋ concentrations was examined. It showed that the algae grown poorly in the culture without Fe^3＋ addition, however, its growth was insignificant difference when Fe^3＋ concentration exceeds 12.5 μmol/L. The proportion of C18：2（n-6）and C18：3（n-6） in total fatty acid was decreased with the increase of Fe^3＋ concentration,but that of DHA was increased.The proportion of PUFA decreased markedly in the range of 12.5 to 120.5 μmol/L Fe^3＋ .The content of DHA and PUFA （dry mass） was increased and then decreased with the increase of Fe^3＋ concertration, the content of DHA achieved the maximum value of 2.4% at 60.5 μmol/L, and that of PUFA reached the highest value of 9.9% at 24.5 μmol/L.

关 键 词：FE^3+ 等鞭金藻 生长 脂肪酸 DHA PUFA 

分 类 号：S968.4[农业科学—水产养殖]