大鼠对氟西泮抗痫耐受和依赖时海马中一氧化氮合酶的变化  

Changes of nitric oxide synthase in hippocampus of rats tolerant to and dependent on the anticonvulsant action of flurazepam

作　　者：张礼萍[1] 王丽[1] 

机构地区：[1]北京大学第一医院儿科,北京100034

出　　处：《中国药理学通报》2005年第8期927-930,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30170994)

摘　　要：目的通过测定大鼠对氟西泮抗痫耐受性和依赖性时海马中一氧化氮合酶(NOS)蛋白表达及活性的变化,探讨一氧化氮(NO)在此过程中可能的作用。方法建立大鼠对氟西泮抗痫耐受性和依赖性的模型。运用免疫印迹及免疫组化法检测海马中NOS蛋白表达,以及比色法检测NOS活性的变化。结果大鼠对氟西泮抗痫耐受组的海马中NOS蛋白表达明显高于对照组;而对氟西泮抗痫依赖组则与对照组差别无统计学意义。两组NOS活性均显著高于各自的对照组。结论NO可能是介导氟西泮抗痫耐受性和依赖性的因素之一。To study the role of nitric oxide by measuring the expression and activity of nitric oxide synthase in hippocampus of rats tolerance and dependence to the anticonvulsant action of flurazepam. Method Animal models of toleran and dependence to flurazepam were established. Nitric oxide synthase in hippocampus was measured using Western blot and immunohistochemistry, and its activity was evaluated using colorimetry. Result Hippocampal nNOS increased in rats tolerance to flurazepam. In rats dependence to flurazepam, hippocampal nNOS increased but without statistical significance. Total NOS activity increased dramatically both in flurazepam tolerane and dependene rats. Conclusion NO may play a role in rats tolerance and dependence to the anticonvulsant action of flurazepam.

关 键 词：氟西泮 耐受性 依赖性 一氧化氮(NO) 一氧化氮 合酶(NOS) 

分 类 号：R-332[医药卫生] R322.81