大豆黄酮血管舒张作用与血管内皮的关系  

Relationship between vasodilatation effect of daidzein and vascular endothelium

作　　者：王红娟[1] 马欣[1] 白宇飞[1] 张小娟[1] 

机构地区：[1]西安交通大学医学院药理学系,陕西西安710061

出　　处：《中国药理学通报》2005年第8期946-949,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30270554)

摘　　要：目的研究植物雌激素大豆黄酮(daidzein,Dai)的舒张血管效应与血管内皮M受体,细胞内、外钙的关系。方法采用家兔离体主动脉环进行等长张力实验。结果Dai(3～100μmol·L-1)对苯肾上腺素(phenylephrine,PHE)依内Ca2+性收缩和依外Ca2+性收缩均具有显著的抑制作用;Dai(10～100μmol·L-1)可使KCl的量效曲线右移,最大效应降低;Dai(10～100μmol·L-1)对ACh引起的血管环的舒张反应有浓度依赖性的增强作用,且在用阿托品阻断M受体后Dai对KCl引起的收缩的舒张效应减弱。结论Dai舒张血管效应与抑制内Ca2+释放和外Ca2+内流有关;Dai对血管环的直接舒张作用与ACh相似,也是通过激动内皮上的M受体释放EDRF等而引起的,且具有内皮依赖性。To study the relationship between the vasodilatation effects of daidzein and M-receptor in the endothelial cell, extracellular Ca^2＋ and intracellular Ca^2＋ Methods Measurement of isometric force of rabbit thoracic aortae rings Dai （ 3 ～100μmol· L^- 1 ） was performed. Results significantly inhibited the contractive response of phenyleohrine dependent on intracellular Ca^2＋ release and extracellular Ca^2＋ inflow ; a shift was produced to the right of the concentrationeffect curve of KCl and the maximum response was depressed by Dai（10～100μmol·L^-1 ） ; Dai（10,30, 100μmol· L^-1 ） enhanced the relaxing effect induced by acetylcholine in a concentration-dependent manner, but the relaxing effect was inhibited after using atropine to block the M-receptor. Conclusion The relaxing effect of Dai was related to the inhibition of the receptor-mediated Ca^2＋-influx and Ca^2＋-release; Dai induced direct relaxing effect by activating the M-receptor in the endothelium and releasing EDRF, which was similar to ACh, and this effect was endothelium-dependent.

关 键 词：大豆黄酮 钙 主动脉 乙酰胆碱 内皮 

分 类 号：R-332[医药卫生] R282.71