激光照射茶树花药培养及其过氧化物酶同工酶的分析  

STUDIES ON LASER-IRRADIATED TEA ANTHER CULTURE AND ANALYSIS ON ITS PEROXIDASE ISOZYME

作　　者：陈震古[1] 刘小刚[1] 许成林 余皖苏 

机构地区：[1]安徽农业大学激光育种研究室

出　　处：《激光生物学》1995年第2期630-635,共6页Acta Laser Biology Sinica

摘　　要：Ｈｅ—Ｎｅ和ＣＯ＿２激光对茶树花药培养的药裂率和出愈率的影响，既有促进作用，也有抑制作用。按出愈率和愈伤组织生长状况的序列：Ｈｅ－Ｎｅ：２０Ｊ／ｃｍ￣２＞ＣＫ＞Ｈｅ－Ｎｅ：３０Ｊ／ｃｍ￣２＞ＣＯ＿２：３５．４Ｊ／ｃｍ￣２。对Ｈｅ－Ｎｅ激光照射花药及其愈伤组织的过氧化物酶同工酶分析，主要表现在弱酶带的变化上，无规律差异。Stimulating and inhibiting effects on frequence of dehiscence and callus on tea anthers were irra-diated with He-Ne and CO￣2 laser occurred in this experiment. In order of anthers callus frequence and callusgrowth are :He-Ne 20J/cm￣2>CK>He-NE30 J/cm￣2>CO_2 35.4J/cm￣2. Analysis of peroxidase isozyme fromanthers and its callus irradiation by He-Ne laser shows that is irregular in the change of weak bands.

关 键 词：激光 茶树花药 愈伤组织 过氧化物酶 同工酶 

分 类 号：S571.103.5[农业科学—茶叶生产加工] S123[农业科学—作物学]