动员自体骨髓干细胞对急性心肌梗死大鼠心功能的影响  

The effect of bone marrow stem cells mobilization on cardiac performance of acute myocardial infarction rats

作　　者：武峰[1] 王海昌[1] 王跃民[2] 李伟杰[1] 张荣庆[1] 秦涛[1] 李虎[1] 

机构地区：[1]第四军医大学西京医院心血管内科,陕西西安710032 [2]第四军医大学基础部生理学教研室,陕西西安710032

出　　处：《心脏杂志》2005年第4期326-329,共4页Chinese Heart Journal

摘　　要：目的:探讨联合应用粒细胞集落刺激因子(G-CSF)和干细胞因子(SCF)动员心肌梗死大鼠的骨髓干细胞并观察对心功能的影响及探讨其可能机制。方法:雄性W istar大鼠40只经结扎冠状动脉前降支制作心肌梗死模型后随机分为两组。①动员组:皮下注射rh-CSF和rh-SCF。②对照组:皮下注射等量生理盐水。28 d后通过测定血流动力学观察大鼠心功能变化,通过伊文氏蓝-TTC染色方法观察心肌梗死范围的变化。通过HE染色方法、免疫荧光标记染色和双重免疫荧光标记染色观察心肌梗死范围内心肌纤维化、血管和心肌再生的变化。结果:动员组心肌组织梗死范围内成纤维细胞增生程度轻,新生血管和心肌细胞的特异蛋白染色阳性,与对照组比较,动员组左室收缩压显著增大(P<0.05),舒张末压显著减小(P<0.05),左室压上升/下降变化最大速率显著增快(P<0.05)。动员组的缺血范围和心肌梗死的范围比对照组均有所缩小(P<0.05)。结论:G-CSF和SCF动员骨髓干细胞可以改善心肌梗死后大鼠的心功能。AIM ： To explore the effect and the possible mechanism of G-CSF and SCF on cardiac function by mobilizing the bone marrow stem cells in acute myocardial infarction rats. METHODS： Myocardial infarction of left ventricle was induced in male Wistar rats by ligation of left coronary artery. The rats （ n = 40 ） were randomly divided into 2 groups： ①The mobilized group receiving hypoderm injection of rhG- CSF and rh-SCF, and ② The control group receiving hypoderm injection of an equivalent amount of normal saline. After 28 days, heart function was examined and hearts were harvested for HE stain, immunofluorescence and double immunofluorescence. Myocardial ischemic size and infarct size were determined by dual staining with Evan＇s blue dye and triphenylterazolium chloride. RESULTS： Compared with the control group, fibrosis was less in the infarct zone of the mobilized group. Regenerative vasculars and myocytes specific proteins were stained positively. Left ventricular systolic pressure, left ventricular enddiastolic pressure and left ventricular pressure decay ± dp/dtmax of the mobilized group were higher than those of the contral group （ P 〈 0.05 ）. There was statistical differences between the two groups. Myocardial ischemic size and infarct size of the mobilized group [ （41±7）% and （43±6）% ] were statistically smaller than those of contral group; [ （53±8）% and （55±10）% ] （P 〈0.05）. CONCLUSION： By using G-CSF and SCF , the heart function of acute myocardial ischemic rats is improved.

关 键 词：骨髓干细胞 自体动员 心肌梗死 急性 

分 类 号：R542.22[医药卫生—心血管疾病]