慢性肝炎患者肝组织胶原半定量测定方法的研究  

Clinical Value of the Method for the Measurement of Collagen in Hepatic Fibrosis Tissue Sections

作　　者：赵治友[1] 汪国运 王金泉[1] 蔡卫民[2] 

机构地区：[1]中国人民解放军第117医院传染病科,浙江杭州310013 [2]浙江大学附属第一医院传染病研究所

出　　处：《中西医结合肝病杂志》2005年第4期204-205,共2页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:探讨慢性肝炎患者肝组织胶原半定量测定方法对肝纤维化量化诊断的临床价值。方法:对40例慢性肝炎和6例正常对照组的肝脏穿刺组织标本,常规组织切片,HE、Masson三色和苦味酸天狼红染色,苦味酸天狼红染色与洗脱液进行比色测定,并观察胶原定量与炎症活动度(G)、炎症半定量计分(SSG)和纤维化分期(S)、纤维化半定量计分(SSS)的相关性。结果:急性肝炎组、慢性肝炎轻度、中度、重度组的G、SSG的计分均值和S、SSS的计分均值呈上升趋势;肝组织切片胶原半定量检测结果,正常对照组为(42.15±1.87)μg/mg、急性肝炎组为(49.04±11.89)μg/mg、慢性肝炎轻度组为(56.24±9.16)μg/mg、慢性肝炎中度组为(87.09±21.81)μg/mg、慢性肝炎重度组为(121.69±10.03)μg/mg,从正常对照组到慢性肝炎重度组胶原半定量也呈上升趋势;且切片胶原含量与其他指标存在较好的相关性,与G、SSG、S、SSS的r分别是0.91、0.89、0.97、0.89,其P均<0.01。结论:肝脏组织切片胶原测定方法可客观反映肝脏纤维化的程度,而且是一个简单而且敏感的量化诊断指标。Objective： To study clinical value of the method for measurement collagen in hepatic fibrosis tissue sections. Methods： Biopsied liver specimen of 40 cases with acute and chornic hepatitis and 6 control cases were made with histological technique included HE stain, Masson stain, Picric acid-Sims red stain technique.After the sections were stained with Picric acid-Siruws red and Fast green simultaneously, the samples were transferredtest tubes and eluted with NaOH- methanol.The absorbances obtained at 540nm and 605nm can be used to determine the amount of collagen and protein. Quantative and semi-quantative scorings were determined according to inflammatory and fibrosis. Results： Liver histology： Inflammation grade （G）, fibrosis stage （S）, semi-quantative inflammatory scoring grade （SSG）, semi-quantative fibrosis scoring stage （SSS） for acute hepatitis, mild, middle, severe chronic hepatitis groups rised increasingly, and collagen content in sections was （ 49.04 ± 11.89） μg/mg, （56.24±9.16） μg/mg, （87.09±21.81）μg/mg, （121.69± 10.03）μg/mg, respectively. The differences of collagen content in sections among these groups were significant, F = 31.77, P 〈 0.01. There was good correlationship between collagen content in sections and G, SSG, S, SSS. The coefficient “r” was 0.91, 0.89, 0.97, 0.89, respectively, P 〈 0.01.Conclusion： These results suggest that measurement collagen in hepatic fibrosis tissue sections might not only accurately reflect objectively the degree of hepatic fibrosis, but also may be a simple, sensitive and quantative method for diagnosis in hepatic fibrosis.

关 键 词：肝纤维化 诊断 肝组织胶原定量 慢性肝炎患者 肝组织切片 胶原含量 半定量 测定方 慢性肝炎重度 正常对照组 

分 类 号：R575.1[医药卫生—消化系统]