嗅鞘细胞对神经干细胞增殖分化的影响  

Effects of OECs on the proliferation and differentiation of NSCs

作　　者：唐洲平[1] 王萍 康慧聪[1] 雷霆[1] 朱遂强[1] 王伟[1] 方思羽[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院临床神经研究中心,武汉430030 [2]湖北恩施自治州中心医院麻醉科

出　　处：《中华实验外科杂志》2005年第9期1123-1124,共2页Chinese Journal of Experimental Surgery

摘　　要：目的研究嗅鞘细胞(OECs)和神经干细胞(NSCs)分别及共培养的相互作用,为CNS神经损伤修复提供移植的理论依据。方法分离培养NSCs和OECs,在无血清DMEM/F12液中NSCs和OECs共培养,用免疫细胞化学和激光共聚焦显微术来分析结果。结果共培养7d观察OECs能促进NSCs增殖分化,分化为神经元、星形胶质细胞和少突胶质细胞,其中神经元比例高达78.2%。结论OECs可促进NSCs的增殖分化,其分化的神经元比例高。Objective To research the interaction between NSCs and OECs when cultured or cocultured respectively to provide the theoretical basis for the repair of CNS damage. Methods NSCs and OECs were separated and cultured, NSC,s and OECs were co-cultured with DMEM/F12 solution, The results were analyzed with immunocyitochemistry method and confocal laser scanning microscopy. Results On the 7th day of co-culture, it was found OECs could promote the proliferation and differentiation of NSCs into neurons, astrocytes and ligodendrocytes. The rate of neurons reached 78.2 %, Conclusion OECs can promote the proliferation and differentiation of NSCs, The rate of neurons is high.

关 键 词：嗅鞘细胞 神经干细胞 共培养 增殖 分化 细胞增殖分化 激光共聚焦显微术 OECS NSCS 免疫细胞化学 

分 类 号：R741[医药卫生—神经病学与精神病学]