CAR抑制卵巢癌细胞株SKOV3侵袭转移表型的研究  

Inhibitory Effect of Coxsackie Adenovirus Receptor on Invasive and Metastatic Phenotype of Ovarian Cancer Cell Line SKOV3

作　　者：王蓓蓓[1] 陈刚[1] 李辅军[1] 周剑锋[1] 卢运萍[1] 马丁[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肿瘤生物医学中心,湖北武汉430030

出　　处：《癌症》2005年第9期1054-1058,共5页Chinese Journal of Cancer

基　　金：国家杰出青年基金(No.30025017);国家(937)重大基础研究项目(No.2002CB513107)~~

摘　　要：背景与目的:柯萨奇-腺病毒受体(coxsackieandadenovirusreceptor,CAR)最早作为2,5型腺病毒的受体而被人们发现和认识,近年的研究发现它还是粘附分子家族的一员,并参与肿瘤恶性表型改变。本研究通过转染真核表达载体,探讨其对卵巢癌细胞株SKOV3侵袭转移表型的影响。方法:利用Westernblot和RT-PCR检测4株卵巢癌细胞CAR的表达;脂质体介导转染含有全长CAR基因的真核表达质粒至SKOV3细胞,经G418筛选出稳定表达的阳性细胞克隆;体外粘附实验及软琼脂克隆形成实验验证CAR对SKOV3侵袭转移表型的影响。结果:4株卵巢癌细胞中,CAR表达水平不同程度下调,其中SKOV3细胞CAR表达缺失。转染后,SKOV3细胞CARmRNA和蛋白质水平表达均明显增高;细胞间粘附力明显增强;软琼脂克隆实验显示转染细胞每孔克隆形成数(25.3±8.9)明显低于未转染组(88.8±14.0)和转染空质粒组(82.5±19.4)。结论:外源性CAR基因的导入可以增强卵巢癌细胞SKOV3间的粘附性,从而抑制肿瘤细胞的侵袭转移表型。BACKGROUND ＆ OBJECTIVE： Coxsackie and adenovirus receptor （CAR） was originally identified as the cellular receptor of 2,5-type adenovirus. Recent researches found that CAR is a subordinate cell adhesion molecule and plays an important role in the malignant phenotype changes of tumor cells. This study was to explore inhibitory effect of CAR on invasive and metastatic phenotype of ovarian cancer cell line SKOV3. METHODS. The expression of CAR in ovarian cancer cell lines SKOV3, CAOV3, SW626, and A2780 was detected by quantitative reverse transcription-polymerase chain reaction （RT-RCR） and Western blot. SKOV3 cells were transfected with the eukaryotic expression plasmid containing full-length CAR cDNA. The positive clones were screened by G418, and identified by RT-PCR, Western blot and ADV/GFP infection assay. The biological behavior changes of positively transfected cells were assessed by colony formation assay and cell adhesion assay. RESULTS. The expression of CAR was down-regulated in the 4 ovarian cancer cell lines, among which SKOV3 cells had no CAR expression, The mRNA and protein levels of CAR were obviously higher in CAR- transfected SKOV3 cells than in untransfected cells and mock-transfected cells; the adhesion ability of CAR-transfected SKOV3 cells was obviously enhanced. The colony formation count was significantly lower in CAR-transfected SKOV3 cells than in untransfected cells and mock-transfected cells （25.3±8.9 vs. 88.8±14.0, 82.5±19.4, P〈0.05）. CONCLUSION. Transfected CAR gene may enhance the adhesion ability of ovarian cancer SKOV3 cells, therefore, inhibit the malignant metastatic phenotype of SKOV3 cells.

关 键 词：卵巢癌/病理学 SKOV3细胞株 柯萨奇-腺病毒受体 侵袭 转移 

分 类 号：R737.31[医药卫生—肿瘤]