VEGF-A和VEGF-C在乳腺癌组织中的表达及其意义  

Expression of Vascular Endothelial Growth Factor A and C in Human Breast Cancer and Their Significance

作　　者：胡帅尔[1] 张雅洁[1] 崔玉梅[1] 张惠球[1] 

机构地区：[1]广州医学院病理教研室,广东广州510182

出　　处：《癌症》2005年第9期1076-1079,共4页Chinese Journal of Cancer

基　　金：广东省科技厅重点引导项目(2004B34001013)~~

摘　　要：背景与目的:VEGF家族都与血管生成相关,血管内皮生长因子-A(vascularendothelialgrowthfactorA,VEGF-A)和血管内皮生长因子-C(vascularendothelialgrowthfactorC,VEGF-C)与肿瘤的生长和转移关系密切。本研究探讨乳腺癌组织中VEGF-A、VEGF-C的表达与癌细胞增殖、微血管密度(microvesseldensity,MVD)和淋巴结转移的关系。方法:采用免疫组织化学方法观察98例乳腺癌组织中VEGF-A、VEGF-C、增殖细胞核抗原(proliferatingcellnuclearantigen,PCNA)、CD34的表达情况。结果:98例乳腺癌组织中,VEGF-A阳性率为85.7%(84/98),VEGF-C阳性率90.8%(89/98),两者在淋巴结转移组表达均高于未转移组,差异具有显著性(P<0.05)。PCNA的表达随着VEGF-A、VEGF-C表达强度增强,肿瘤细胞增殖活性也随之增强(r=0.432,P=0.000;r=0.294,P=0.001)。淋巴结转移组MVD值(64.26±26.40)明显高于未转移组(50.29±29.35)(P<0.05),且随着VEGF-A表达增强,MVD也随之增高(r=0.327,P<0.001),VEGF-C表达与MVD无相关性(r=0.123,P>0.05)。结论:VEGF-A主要介导了血管生成、细胞增殖和转移;VEGF-C促进乳腺癌细胞增殖,与血管密度无关,与淋巴结转移密切相关。BACKGROUND ＆ OBJECTIVE, Vascular endothelial growth factor （VEGF） family is related to angiogenesis. VEGF-A and VEGF-C are closely related to tumorigenesis and lymphatic metastasis. This study was to detect the expression of VEGF-A and VEGF-C in breast cancer, and explore their correlation to cell proliferation, microvessel density （MVD）, and lymphatic metastasis of breast cancer. METHODS. The expression of VEGF-A, VEGF-C, proliferating cell nuclear antigen （PCNA）, and CD34 in 98 samples of breast cancer were detected by SP immunohistochemistry. RESULTS. The positive rate of VEGF-A was 85.7%; that of VEGF-C was 90.8%. The positive rates of VEGF-A and VEGF-C were significantly higher in cancers with lymph node metastasis than in cancers without lymph node metastasis （P〈0.05）. The positive rate of PCNA was 98.0%; its expression level was increased with the expression levels of VEGF-A and VEGF-C （r= 0.432, P=0.000; r=0.294, P=0.001）. MVD was significantly higher in cancers with lymph node metastasis than in cancers without lymph node metastasis （64.26±26.40 vs. 50.29±29.35, P〈0.05）. MVD was positively correlated to the expression of VEGF-A （r=0.327, P〈0.001）, but had no correlation to VEGF-C （r=0.123, P〉0.05）. CONCLUSIONS; VEGF-A mainly mediates angiogenesis, cell proliferation, and metastasis of human breast cancer. VEGF-C promotes cell proliferation of human breast cancer; it is correlated to lymph node metastasis, but has no correlation to MVD.

关 键 词：乳腺癌/病理学 VEGF-A VEGF-C 细胞增殖 肿瘤转移 

分 类 号：R737.9[医药卫生—肿瘤]