职业性三苯接触对人外周血淋巴细胞染色体结构的影响  

Research on the Relationship between Occupational Exposure to Mixed Benzene and Chromosomal Aberration of Peripheral Blood Lymphocytes

作　　者：高峰 王宝梅[2] 梁玉珍[3] 徐真[2] 李秀梅[2] 王晓华[2] 

机构地区：[1]河北邯郸市环保局,河北邯郸056001 [2]河北工程学院医学部,河北邯郸056029 [3]河北省邯郸市第三医院,河北邯郸056001

出　　处：《环境与职业医学》2005年第4期359-360,共2页Journal of Environmental and Occupational Medicine

摘　　要：[目的]探讨长期接触混苯对人外周血淋巴细胞染色体结构的影响。[方法]用细胞遗传学方法对78名混苯作业工人(接触组)和78名食品加工厂工人(对照组)进行外周血淋巴细胞染色体畸变、姊妹染色单体互换、微核率进行测定。[结果]接触组染色体畸变、总畸变率及细胞畸变率均高于对照组(P<0.01),畸变类型以染色体裂隙和断裂为主, 而两组的非整倍体检出率、姊妹染色体单体互换差别均无显著性(P>0.05)。接触组无论是微核率和微核细胞率均高于对照组(P<0.001)。同时发现,饮酒可能增加混苯的吸收,进而增加对混苯接触者的遗传毒性。[结论]职业性混苯接触可引起人外周血淋巴细胞染色体畸变。[ Objective ] To study the effect on chromosomal structure of peripheral blood lymphocytes in workers exposed to mixed benzene for long time. [ Methods ] With method of cellular genetics to analyze chromosomal aberration of peripheral blood lymphocytes, sister chromatid exchange and micronucleus rate in 78 mixed-benzene-exposed workers （ exposure group ） and 78 foodstuff workers （ control group ）. [ Results ] Chromosomal aberration rate, overall aberration and cellular aberration in exposure group were higher than those in the control group （ P 〈 0.01 ）. The major types of aberration were chromosome gap and breakage, but the difference in both rates of aneuploidy and sister chromatid exchange between two groups were not statistically significant （ P 〉 0.05 ）. Micronucleus rate and micronucleus cell rate of the exposure group were higher than those in the control group （ P 〈 0.01 ）. At the same time, intaking alcohol might be. a fact, or which would aggravate the genetic toxicity of benzene. [ Conclusion ] Occupational exposure to mixed benzene may induce chromosomal aberration of peripheral blood lymphocytes.

关 键 词：淋巴细胞 混苯 遗传毒性 染色体畸变 

分 类 号：R114[医药卫生—卫生毒理学]