凝血酶介导大鼠脑组织细胞间黏附分子-1表达与白细胞浸润的研究  

Expression of intercellular adhesion molecule-1 and neutrophil infiltration induced by injection of thrombin in rats

作　　者：高长越[1] 周华东[1] 陈曼娥[1] 

机构地区：[1]第三军医大学大坪医院神经内科,重庆400042

出　　处：《中国危重病急救医学》2005年第9期552-554,共3页Chinese Critical Care Medicine

基　　金：全军医药卫生科研基金课题(01MB107)

摘　　要：目的探讨凝血酶在脑出血后血肿周围区炎症反应中的作用。方法72只Wistar大鼠随机分为假手术对照组和凝血酶模型组,分别用免疫组化和髓过氧化物酶(MPO)法检测注入凝血酶后6、12、24、48、72和120h不同时间点脑血肿周围组织细胞间黏附分子1(ICAM1)表达及白细胞浸润情况。结果注入凝血酶后6h脑血肿周围组织ICAM1表达即已升高,于72h达到高峰,持续至120h仍有较高表达;而该区组织MPO活性于6h即已开始升高,24h达一高峰,48h有所下降,至72h达最高峰,持续到120h活性仍较高。MPO与ICAM1的表达除24h略不一致外,其余各时间点二者均一致,呈明显正相关。结论凝血酶可诱导局部脑组织表达ICAM1,并与该区域白细胞的浸润明显相关;凝血酶在脑出血后血肿周围组织的继发性损伤过程中起着重要作用。Objective To investigate the role of the thrombin in reducing inflammatory response m brain tissue adjacent to hematoma in the brain. Methods Seventy-two male Wistar rats were randomly divided into two groups： sham-operated control group and thrombin injection group. Immunohistochemical technique and myeloperoxidase （MPO） determination were respectively used to evaluate intercelludar adhesion molecule - 1 （ICAM-1） expression and neutrophil infiltration in adjacent brain tissue of the thrombin injection zone at 6, 12, 24, 48, 72 and 128 hours after thrombin injection. Results The expression of ICAM-1 in tissues adjacent to thrombin injection started to increase 6 hours after the injection, peaking at 72 hours, and lasting for 120 hours. MPO activity of tissues in the area increased 6 hours, reached a high level at 24 hours, then decreased slightly, and it peaked at 72 hours after thrombin injection, correlating closely with the increase in ICAM-1 expression except the changes at 24 hours. Conclusion The increased expression of ICAM-1 after thrombin injection is correlated with the degree of the neutrophil infiltration in the brain tissue around the thrombin injection zone, indicating thrombin is important to produce secondary brain damage after cerebral hemorrhage.

关 键 词：凝血酶 细胞间黏附分子-1 白细胞浸润 脑出血 大鼠脑组织 ICAM-1表达 酶介导 血肿周围组织 Wistar大鼠 MPO活性 

分 类 号：R743.34[医药卫生—神经病学与精神病学]