肌苷对缺血再灌注脑组织VCAM-1表达的影响  

EFFECTS OF INOSINE ON EXPRESSION OF VCAM-1 IN CEREBRA AFTER ISCHEMIA AND REPERFUSION

作　　者：边立忠[1] 刘成玉[2] 赵桂荣[1] 陈兰亭[1] 姜训忠[1] 李庆之[1] 

机构地区：[1]淄博市齐都医院器官移植中心,山东淄博255400 [2]青岛大学医学院诊断学教研室

出　　处：《齐鲁医学杂志》2005年第4期331-332,334,共3页Medical Journal of Qilu

摘　　要：①目的探讨肌苷对局灶性缺血再灌注后脑组织血管细胞间黏附分子-1(VCAM-1)表达的影响.②方法将健康成年雌性SD大鼠70只随机分为假手术组、治疗组和对照组,治疗组在缺血再灌注前腹腔注射肌苷注射液,另两组腹腔注射相同体积的生理盐水.后两组分别在脑缺血90 min后再灌注2 h、6 h、12 h、1 d、2 d、3 d、7 d和14 d等不同时间,用免疫组化技术观察VCAM-1的表达.③结果在皮质区,VCAM-1的表达在缺血再灌注不同时间点呈现不同水平,假手术组和对照组缺血再灌注2 h之前有痕量表达,VCAM-1的阳性表达在缺血再灌注6 h有较明显增加,1～2 d时表达达高峰,之后逐渐下降,到7 d时VCAM-1的表达接近假手术组水平;在纹状体区呈现与皮质区相似的表达规律,但其表达与同时间点皮质区比较水平更高.在治疗组VCAM-1的表达规律类似于对照组,在同一时间点较对照组表达显著降低,又明显高于假手术组.④结论适量输注肌苷可能通过VCAM-1作用机制对缺血再灌注脑组织起一定的保护作用.Objective To study the effects of inosine on the expression of vascular cell adhesion molecule 1（VCAM-1） in cerebra after focal cerebral ischemia and reperfusion. Methods Seventy adult female Sprague Dawley（SD） rats were randomly divided into sham-operation group （SG）, and ischemia-reperfusion group（IG）. Both SG and IG groups were subdivided into eight subgroups, The rats were made into middle cerebral artery occlusion （MCAO） model at 2 h, 6 h, 12 h, 1 d, 2 d, 3 d, 7 d and 14 d, respectively, after ischemia of 90 min. The expression of VCAM-1 was measured by immunohistochemistry （IHC）. Results In IG, the level of ICAM-1 in cerebral cortex and striatum varied with the time of ischemia reperfusion. The trace expression of VCAM-1 occurred at 2 h, marked at 6 h, peak at 1-2 d and then decreased gradually, whereas they were hardly observed in SG. In TG, although the level of VCAM-1 also varied with the hours of ischemia-reperfusion, the number of cerebral positive cells was fewer than corresponded hours in IG and more notable than in SG. There was a significant difference between the IG and the TG. Conclusion Appropriate concentration of inosine might be beneficial to cerebral tissue after ischemia reperfusion.

关 键 词：脑缺血 再灌注损伤 血管细胞间黏附分子-1 肌苷 免疫组织化学 

分 类 号：R364.12[医药卫生—病理学]