黄单胞菌L4毒素对反枝苋线粒体及细胞壁和表皮气孔的影响  

Effect of Xanthomonas L4 Phytotoxin on Mitochondrial Stoma and Cell Wall of Amaranthus Retroflex(Redroot Amarant)

作　　者：孙自玲[1] 李永泉[2] 李明智[2] 徐凌[2] 

机构地区：[1]苏州大学基础医学系生物化学与分子生物学教研室,江苏苏州215007 [2]浙江大学生命科学院,浙江杭州310027

出　　处：《江苏大学学报（医学版）》2005年第4期285-287,共3页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(30370939);浙江省自然科学基金资助项目(300054)

摘　　要：目的:通过观察黄单胞菌L4毒素对反枝苋线粒体、细胞壁和表皮气孔叶片细胞的影响来探讨毒素的作用机理。方法:采用M itach i-800透射电镜和Ph ilips-XL30-ESEM扫描电镜方法观察。结果:黄单胞菌L4毒素使反枝苋叶片的线粒体膨大,破坏线粒体内膜结构,引起细胞壁质分离,叶面气孔关闭。结论:黄单胞菌L4毒素破坏线粒体内膜结构,从而使ATP代谢不能正常进行,叶面气孔关闭,而且蒸腾作用的热量无法散发,使叶片坏死萎蔫。Objective： To observe effects of Xanthomonas LA phytotoxin on mitochondrial, stoma and cell wall in order to study the mechanism of herbicide. Methods： To do through transmission electron microscope（TEM） and scanning electron microscope（SEM）. Results： It was founded that cristae of mitochondria was swelled after treated 2 h . then cell membrane was disrupted, with mitochondria vesiculated and stoma closed. Heat of transpiration cannot be emitted and induce leaf to wilt. Conclusion： Xanthomonas LA phytotoxin can disrupt mitochondrial to inhibit oxidation and phosphorylation to couple with each other, so ATP can not be formed normally result in leaves to death.

关 键 词：黄单胞菌L4毒素 线粒体 细胞壁 气孔 

分 类 号：X17[环境科学与工程—环境科学]