两种不同乳腺癌细胞系组织因子表达与细胞转移力比较研究  

A Study on Tissue Factor Expression and Cell Metastatic Ability in Two Breast Carcinoma Cell Lines

作　　者：郭艳丽[1] 宋善俊[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院血研所,湖北武汉430022

出　　处：《中国医师杂志》2005年第9期1187-1188,共2页Journal of Chinese Physician

摘　　要：目的探讨两种不同乳腺癌细胞系MCF-7和MDA-MB231组织因子(tissuefactor,TF)表达差异,揭示TF与肿瘤侵袭转移力关系。方法用RT-PCR法检测两乳腺癌细胞系MCF-7和MDA-MB231TFmRNA表达,用流式细胞术检测两种细胞系表面TF表达,用改良Bodyen小室法研究细胞侵袭转移力,t检验差异性。结果MDA-MB231细胞TFmRNA和抗原表达明显高于MCF-7细胞,而且MDA-MB231细胞侵袭转移力高于MCF-7,差异显著。结论TF表达与乳腺癌细胞转移能力成正比,可望成为肿瘤治疗一个靶点。Objective To study the relationship between tissue factor expression and cell metastatic ability in two breast carcinoma cell lines. Methods RT-PCR and flow cytometry（FCM） were used to detect the mRNA and protein expressions of tissue factor（TF） in the breast carcinoma cell lines MCF-7 and MDA-MB231, respectively. The metastatic ability of the two breast carcinoma cell hnes was determined using modified Boyden chambers. The data were analyzed by t test. Results The mRNA and protein expression levels of TF in MDA-MB231 were obviously higher than those in MCF-7, and the metastatic ability of MDA-MB231 was stronger than that of MCF-7, which was significantly different between the two cell lines. Conclusion TF expression was positively related with breast carcinoma metastatic ability, and TF may be a therapeutic target for breast carcinoma.

关 键 词：组织因子 乳腺癌 转移 乳腺癌细胞系 转移力 MCF-7细胞 流式细胞术检测 factor mRNA表达 PCR法检测 

分 类 号：R737.9[医药卫生—肿瘤]