二氢石蒜碱对家兔脑血管的选择性作用特点(英文)  

Selective effect of dihydrolycorine on cerebral vessels of rabbits

作　　者：蓝星莲[1] 潘龙瑞[1] 龚新荣[1] 龚应霞[1] 任世兰[1] 于龙顺[1] 张秋芳[1] 赵国举[1] 

机构地区：[1]郧阳医学院药理教研室,湖北省十堰市442000

出　　处：《中国临床康复》2005年第29期202-204,共3页Chinese Journal of Clinical Rehabilitation

基　　金：湖北省自然科学基金资助项目(200J166)~~

摘　　要：背景:二氢石蒜碱在外周能抑制交感神经末梢释放儿茶酚胺,阻断α,β肾上腺素能受体,具有扩血管、降血压,抗缺糖、缺氧作用。目的:应用重酒石酸去甲肾上腺素和KCl引起血管收缩反应,观察二氢石蒜碱对家兔脑基底动脉、胸主动脉及心室乳头肌的选择性作用。设计:观察性对比实验。单位:郧阳医学院药理教研室。材料:实验于2001-03/07在郧阳医学院药理教研室的科研室进行,选择46只成年健康新西兰白兔。方法:新西兰白兔在麻醉下耳缘静脉注射10mL空气处死,迅速取出脑、胸主动脉及心脏。将基底动脉、胸主动脉制成4.0～5.0mm的血管环,打开心脏分离心室乳头肌,分别连接张力换能器。实验观察:①记录重酒石酸去甲肾上腺素或KCl单用时收缩曲线的变化:胸主动脉给予重酒石酸去甲肾上腺素0.1mmol/L或KCl60mmol/L引起血管亚最大收缩,待收缩曲线平稳后,分别用累积法加入不同浓度的二氢石蒜碱或尼莫地平。基底动脉给予重酒石酸去甲肾上腺素0.1mmol/L或KCl60mmol/L,待血管收缩达峰值后,冲洗液每20min换1次,共3次。再用不同浓度二氢石蒜碱或尼莫地平,20min后再加入重酒石酸去甲肾上腺素或KCl,待曲线平稳后,观察每单剂药物所致收缩曲线的变化。②记录电刺激心室乳头肌时收缩的幅度变化:1次/s,波宽3ms,阈电压的120%强度电刺激以引发心室乳头肌同步收缩活动,待收缩曲线平稳后,分别用累加法加入二氢石蒜碱或尼莫地平。主要观察指标:①离体基底动脉、胸主动脉及心室乳头肌收缩张力变化。②二氢石蒜碱或尼莫地平对重酒石酸去甲肾上腺素或KCl所致家兔血管环收缩的半数有效浓度值。结果:46只家兔离体基底动脉、胸主动脉及心室乳头肌标本均进入实验分析。①二氢石蒜碱使基底动脉静息张力升高,尼莫地平则使其降低,对胸主动脉无影响。②重酒石酸去甲肾上腺素�BACKGROUND： Dihydrolycorine （DL） can inhibit the peripheral release of catecholamine from sympathetic nerve ending and block α, β adrenalinergic receptor. It has multiple pharmacological actions, such as vascular dilation, hypotension, anti-hypoglycemia and anti-ischemia. OBJECTIVE： To investigate the selective effect of DL on basilar artery, thoracic aorta and ventricular papillary muscle of rabbit by means of observing the vasoconstriction induced by noradrenaline bitartrate and KCl. DESIGN： Observation of comparative experiment. SETTING： Pharmacological Department of Yunyang Medical College. MATERIALS： This study was carried out at Pharmacological Department of Yunyang Medical College between March and July 2001, and 46 adult healthy New Zealand rabbits were selected. METHODS： New Zealand rabbits were put to death by intravenous injection of 10 mL air from the vein of aural edge, and soon after death, brain, thoracic aorta and heart were obtained. Basilar artery and thoracic aorta was linked into 4.0-5.0 mm vascular rings; meanwhile, ventricular papillary muscle were separated and connected with tension transducer. Observations：① Changes of vasoconstriction curve due to administration of single dose of noradrenaline bitartrate or KCl：Sub-maximal constriction of thoracic aorta was induced by 0.1 mmol/L noradrenaline bitartrate or W60 mmol/L KCl, and when constriction curve became stable, DL or nimodipine of different dosage was added. Basilar artery was exposed to 0.1 mmol/L noradrenaline bitartrate or 60 mol/L KCl, and rinsing fluid was changed once every 20 minutes and thrice in all when vasocontratcion reached the peak level, then different dosage of DL or nimodipine was added 20 minutes later, and the changes of vasoconstriction curve due to administration of single dose of noradrenaline bitartrate or KCl were observed when constriction curve became stable. ② Changes of amplitude of constriction of ventricular papillary muscular induced by electro-stimulation： Electro-stimulation

关 键 词：脑 基底动脉/药物作用 剂量效应关系 药物 去甲肾上腺素 石蒜碱 主动脉 胸 心室 乳头肌/药物作用 

分 类 号：R743[医药卫生—神经病学与精神病学]