挤压伤后脊髓腹角和背角中神经营养因子-3和神经营养因子-4表达的时间演变规律(英文)  被引量:1

Time variation of the expressions of neurotrophin-3 and neurotrophin-4 in ventral and dorsal horns of spinal cord after crushed injury

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作  者:董晓霞[1] 张小平[2] 李明[2] 李雪雁[2] 

机构地区:[1]北京联合大学继续教育学院,北京市100000 [2]昆明医学院解剖教研室,云南省昆明市650031

出  处:《中国临床康复》2005年第29期225-227,共3页Chinese Journal of Clinical Rehabilitation

基  金:云南省自然科学基金(2000C0057M);云南省教委基金资助(0012012)~~

摘  要:背景:神经营养因子-3和神经营养因子-4对体内、外神经元的发育、存活及功能维持具有重要的作用,是否对在挤压伤后脊髓神经元有保护和修复作用?目的:观察脊髓挤压伤模型大鼠伤后不同时间脊髓腹角和背角神经元神经营养因子-3和神经营养因子-4的表达,并分析其变化规律。设计:随机对照实验,单因素方差分析。单位:北京联合大学继续教育学院,昆明医学院人体解剖学教研室。材料:实验于2003-01/03在昆明医学院神经科学研究所完成,选择成年SD大鼠24只,随机分为4组,对照组,挤压伤24h组,挤压伤7d组及挤压伤21d组,每组6只。方法:挤压伤各组大鼠麻醉后,在T11行椎板切开后挤压大鼠T13脊髓节段,分别于术后24h,7d及21d断头处死动物,迅速取脊髓L1-L3节段制作20μm厚冰冻横切片。对照组不进行任何处理,和挤压伤24h组同时间点处死大鼠,制备切片过程相同。观察神经营养因子-3和神经营养因子-4样免疫反应阳性细胞在成年大鼠脊髓腹角和背角的分布,并计数两者相同面积内腹角和背角阳性有核细胞数。主要观察指标:①神经营养因子-3和神经营养因子-4在各组大鼠脊髓腹角和背角的分布。②神经营养因子-3和神经营养因子-4在各组大鼠脊髓腹角和背角神经元数量。结果:24只大鼠全部进入结果分析。①神经营养因子-3免疫阳性反应物主要在胞核着色,神经营养因子-4则胞浆及胞核均着色。②各组大鼠脊髓腹角和背角神经营养因子-3阳性神经元数量:挤压伤7d组和挤压伤21d组腹角阳性神经元数明显高于对照组和挤压伤24h组犤10.2±1.1,11.4±3.2,6.2±1.8,7.4±2.4,(P<0.01)犦;背角阳性神经元数仅挤压伤21d组高于对照组犤86.4±9.8,71.3±8.3,(P<0.01)犦,而挤压伤24h组及7d组低于对照组犤48.5±5.1,41.5±3.7,71.3±8.3,(P<0.01)犦。③各组大鼠脊髓腹角和背角神经营养因子-4阳性神经元数量:挤压伤24h组,挤�BACKGROUND: Neurotrophin-3 (NT3) and neurotrophin-4 (NT4) play an important role in the growth, survival and functions of neurons in vivo or in vitro. However, it has not been reported whether they can protect and repair neurons of spinal cord after crushed spinal cord injury (cSCI). OBJECTIVE: To explore the expression of NT3 and NT4 in ventral and dorsal horns of spinal cord at various time points after cSCI and analyze the pattern of expression changes. DESIGN: Randomized controlled study and single analysis of variance. SETTING: Continuing Education College of Beijing Union University; Department of Anatomy, Kunming Medical College. MATERIALS: The experiment was conducted in the Institute of Neuroscience of Kunming Medical College from January to March 2003. Totally 24 adult SD rats were divided randomly into 4 groups: control group, 24-hour eSCI group, 7-day cSCI group, and 21-day cSCI group with 6 rats in each group. METHODS: After anesthesia, rats in each cSCI group were dissected at vertebra TH and crushed at spinal cord T13. After 24 hours, 7 days and 21 days, the rats were killed respectively by cutting their heads off, and spinal cord L1-L3 was taken out to make frozen transactional slices of 20μm. Rats in control group received no treatment and were killed at the same time point as those in 24-hour cSC1 group, and the process of slice making was the same as mentioned above. The distribution of NT3 and NT4 positive cells in the spinal cord ventral and dorsal horns of adult rats was observed, and NT3 and NT4 positive nucleus in the ventral and dorsal horns of the same areas were counted. MAIN OUTCOME MEASURES:① Distribution of NT3 and NT4 in the rats' spinal cord ventral and dorsal horns; ② Number of NT3 and NT4 neurons in the rats' spinal cord ventral and dorsal horns. RESULTS: All the 24 rats entered the final analysis. ① NT3 positive products were mainly stained in nucleus while NT4 was stained in cytoplasm and nucleus. ② Number of NT3 neurons in ventral and

关 键 词:脊髓损伤 创伤和损伤 神经组织蛋白质类 

分 类 号:R745[医药卫生—神经病学与精神病学]

 

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