烟草病源蛋白基因导入马铃薯东农303的研究  

INTRODUCTION OF PATHOGENESIS-RELATED PROTEIN GENE INTO POTATO CULTIVAR DONGNONG 303 USING AN AGROBACTERIUM TUMEFACIENS BINARY VECTOR

作　　者：李文芙[1] 双宝[1] 李文滨 陈伊里 田兴亚 吕文河 王凤义 朱祥春 秦昕 杨丽娟[1] 

机构地区：[1]东北农业大学生物工程系,东北农业大学农学系

出　　处：《东北农业大学学报》1996年第1期75-80,共6页Journal of Northeast Agricultural University

基　　金：黑龙江省科委自然基金

摘　　要：研究建立了一套适于我国北方马铃薯主栽品种东农３０３外源基因导入的体系，并成功地将烟草病原蛋白基因导入该品种．结果显示，块茎外植体比其它外植体更适用于基因导入。农杆菌浓度对Ｔ—ＤＮＡ转移有明显影响，５×１０８ＣＦＵ／ｍＬ是较适宜的浓度．组织化学和分子印迹杂交证明，外源基因已经整合入马铃薯染色体组中。试验选出８个转基因个体，将通过无性系繁殖，进一步鉴定遗传稳定性。In search of establishing a system for genetic tansformation of potato, a pathogenesis-related protein gene from tobacco was successfully transferred into potato cultivar Dong Nong 303 via binary vector. According to southern blot analysis and histochemical assay for-glucuronidase, it was concluded that foreign genes was integrated in potato genome and could be expressed in potato tissues. Of three kinds of explants, a highly efficient introduction was obtained from tuber disc.The concentration of Agrbobacterium tumefaciens had a significent interaction on gene transfer. The most suitable concentration was 5 ×108CFU / mL. From present experiment 8 individuals transferred with foreign gene were selectedand expected to estimate genetic stability using somaclones.

关 键 词：马铃薯 外源基因导入 PCR 烟草病原蛋白 

分 类 号：S532[农业科学—作物学] S332.9