一氧化氮对热性惊厥大鼠γ-氨基丁酸B受体亚基表达的影响  

Nitric Oxide Regulated Expression of γ-Aminobutyric Acid B Recepto r Subunits during Recurrent Febrile Seizures

作　　者：韩颖[1] 秦炯[1] 卜定方[2] 杨志仙[1] 常杏芝[1] 杜军保[1] 

机构地区：[1]北京大学第一医院儿科,北京100034 [2]北京大学第一医院中心实验室,北京100034

出　　处：《实用儿科临床杂志》2005年第9期905-907,i0002,共4页Journal of Applied Clinical Pediatrics

基　　金：卫生部临床学科重点项目基金资助(20010912)

摘　　要：目的探讨一氧化氮(NO)对热性惊厥大鼠γ-氨基丁酸B受体(GABABR)亚基表达的影响。方法大鼠随机分为对照组,热性惊厥(FS)组,FS+硝普钠(SNP)组,FS+NG-单甲基-L-精氨酸(L-NMMA)组。采用热水浴诱导大鼠FS,隔日诱导1次,共10次。采用分光光度计法测定大鼠血浆NO含量;原位杂交方法观察GABABR亚基mRNA和c-fos基因表达情况;采用免疫组织化学方法观察GABABR亚基和Fos蛋白表达情况。结果与FS组比较,FS+SNP组血浆NO含量明显升高,而FS+L-NMMA组血浆NO含量明显降低;FS+L-NMMA组GABABR1和GABABR2表达均高于FS组;FS+SNP组GABABR2表达低于FS组,而GABABR1表达与FS组比较无明显改变;SNP的干预使c-fos基因和Fos蛋白表达增强,而L-NMMA的干预使其表达降低。结论NO可通过调节GABABR的功能,在反复FS中发挥作用。Objective To explore the effect of nitric oxide （NO） on γ-aminobutyric acid B receptor （GABABR） subunits during recurrent febrile seizures （FS）. Methods Twenty-four Sprague-Dawley rats aged 21 days were randomly divided into 4 groups： control group （37.0℃ water, n = 8）, FS group （45.2℃ water, n = 8）, FS ＋ SNP group （45.2 ℃ water, n = 8）, FS ＋ L-NMMA group （45.2 ℃ water, n =8）. FS rats were induced 10 times in a warrn-water bath, once every 2 days. The plasma level of NO was detected by the spectrophotometer. The expressions of GABABR subunit mRNA and c-fog gene were examined by in situ hybridization. The expressions of GABABR subunit and Fos protein were observed by immunohistochemistry. Results The plasma level of NO increased in FS ＋ SNP group while decreased in FS＋ L-NMMA group compared with that in FS group. The expressions of GABABR2 were down-regulated in FS＋ SNP group, while GABABR1 hardly changed compared with these in FS group. In FS＋ L-NMMA group, both the expression of GABABR2 and GABABR2 up regulated compared with those in FS group. The expressions of c-fos gene and Fos protein were significantly enhanced after recurrent FS. SNP elevated the expressions of c-los gene and Fos protein, while 1. NMMA down regulated the expressions of them. Conclusion NO may play a regulatory role through modulating GABABR function in the pathogenesis of recurrent FS.

关 键 词：惊厥 发热性 一氧化氮 一氧化氯合酶 γ-氨基丁酸 受体 

分 类 号：R363[医药卫生—病理学]