胃癌FHIT基因表达与肿瘤细胞增殖关系的研究  

Expression of FHIT gene in gastric cancer and relationship with proliferation of tumor cell

作　　者：孟利伟 蔡秀军[1] 

机构地区：[1]浙江大学医学院附属邵逸夫医院普外科

出　　处：《浙江医学》2005年第9期650-651,666,共3页Zhejiang Medical Journal

摘　　要：目的探讨人胃癌组织中脆性组氨酸三联体(FHIT)基因的表达及与肿瘤细胞增殖的关系。方法采用免疫组化法检测60例胃癌组织及其癌旁正常组织中FHIT基因、Ki-67蛋白、p21waf1蛋白的表达。结果(1)胃癌组织中FHIT基因表达水平显著低于癌旁正常组织(P<0.01)。(2)胃癌组织中FHIT基因表达水平与胃癌细胞增殖活性呈显著负相关(P<0.01)。(3)胃癌组织中FHIT基因表达水平降低与p21waf1蛋白的丢失存在显著正相关(P<0.01)。结论FHIT基因作为一种抑癌基因,其表达在胃癌组织中明显下降,与肿瘤细胞增殖过度有关。FHIT基因可能通过p21waf1蛋白参与了对肿瘤细胞周期的调控。Objective To investigate the expression of FHIT gene in gastric cancer and explore the relationship of FHIT gene expression with the proliferation of tumor cell. Methods Immunohistochemistry SP method was used to determine the expression of FHIT gene and Ki-67 protein and p21^wafl protein in 60 gastric cancer samples while 60 corresponding paratumor normal tissues as control. Results （1）The expression of level of FHIT gene was found to be significantly lower in gastric cancer tissues than in paratumor normal tissues （P〈0.01）. （2）The level of FHIT gene expression might be negative related with the proliferation of gastric cancer cell （P〈0.01）. （3）The reduction of FHIT gene expression might be positive related with the reduction of p21^wafl protein in gastric cancer tissue （P〈0.01）. Conclusion As a kind of tumor suppressor gene, the expression of FHIT gene is significantly lower in gastric cancer and is related to the over-prollferation of tumor cell. The FHIT gene might be involved in the regulation of cell cycle control and might be mediated by p21^wafl protein.

关 键 词：胃癌 FHIT基因 细胞增殖 基因表达水平 肿瘤细胞增殖 胃癌组织 脆性组氨酸三联体 P21^WAFL 癌旁正常组织 免疫组化法检测 

分 类 号：R735.2[医药卫生—肿瘤]