诱导小鼠肝硬化对结肠癌肝转移的抵抗作用  

Induced cirrhosis liver is resisitant to liver metastasis from colorectal cancer in mice

作　　者：贾镭[1] 姜达[1] 董倩[1] 邝钢[2] 董稚明[2] 单保恩[2] 

机构地区：[1]河北医科大学第四医院肿瘤内科 [2]河北省肿瘤研究所,河北石家庄050011

出　　处：《中国癌症杂志》2005年第4期317-320,共4页China Oncology

摘　　要：目的:研究诱导小鼠肝硬化对结肠癌肝转移的抵抗作用及其可能的机制。方法:诱导小鼠肝硬化及结肠癌肝转移模型,比较正常肝与肝硬化小鼠肝转移率;免疫组化法测定层粘连蛋白(laminin,LN)、纤维连接蛋白(fibronectin,FN)及E-选择素(E-selectin)在小鼠肝脏的表达。结果:①肝硬化小鼠肝转移率(4/16,25%)低于正常肝小鼠(14/16,87.5%)(P=0.001);②肝硬化后移植瘤小鼠LN、FN表达高于正常肝移植瘤小鼠,E-selectin表达低于正常肝移植瘤小鼠。结论:动物实验证实肝硬化对结肠癌肝转移有抵抗作用;LN、FN及E-selectin在此过程中可能起一定作用。Purpose： To study the resistance of induced mice cirrhosis to liver metastasis from coloreetal cancer and its possible mechanisms.Methods： Developed a mouse model of liver eirrhosis and liver metastases from colorectal cancer,observed the incidence of liver metastasis in cirrhosis liver mice and normal liver mice; examined the expressinn of liminin （LN）,fibronectin（FN） and E-selectin in livers hy immmohistochemistry（IH）.Results： The incidence of liver melustasis in cirrhotic mice（4 of 16,25%）was significantly lower（P=0.001） than that in normal liver mice（14 of 16.87.5%）.The expression of liminin,fibronectin in eirrhosis mice were significantly higher than that in normal liver mice inoculated with tumor,the expression of E-selectin in cirrhotie mice were signifieantly higher than that in normal liver mice inoculated with tumor.the expression of E-selectin in cirrhotic mice was signifieatly lower than that in normal liver mice imoculated with tumor.Conclusions： It was comfirmed that cirrhotic liver is resistant to the development of liver metastasis from colorectal cancer through animal experiments.There were eridence that liminin.fibronectin and E-selectin played important roles in the process.

关 键 词：转移 肝硬化 肝转移 纤维连接蛋白 层粘连蛋白 E-选择素 BABL/C小鼠 

分 类 号：R735.35[医药卫生—肿瘤] R73-37[医药卫生—临床医学]