端粒、端粒酶及端粒酶催化亚基在大肠癌中的作用  

Role of telomere, telomerase and hTERT in colorectal carcinoma

作　　者：刘剑仑[1] 葛莲英[1] 黎丹戎[1] 张贵年[1] 

机构地区：[1]广西医科大学附属肿瘤医院胃肠乳腺外科,广西南宁530021

出　　处：《中国癌症杂志》2005年第4期362-364,共3页China Oncology

基　　金：广西卫生厅资助项目(9954)。

摘　　要：目的:探讨端粒、端粒酶活性及端粒酶催化亚基蛋白(hTERT)在大肠癌发生发展、侵袭转移中的作用。方法:应用Southern blot、端粒重复序列扩增(TRAP)和免疫组织化学方法检测端粒长度(terminal restrictionfragments,TRFs)、端粒酶活性及hTERT表达水平。结果:大肠癌TRFs明显缩短,且随大肠癌Dukes分期的进展进一步缩短;端粒酶活性及hTERT在大肠癌组织中的阳性表达率分别为83.33%及76.67%,显著高于其他组织(P<0.05);大肠癌组织端粒酶与淋巴结转移关系密切,伴淋巴结转移大肠癌组织中的端粒酶活性及hTERT阳性表达率为80%和70%,显著高于无淋巴结转移者的0%和5%(P<0.05);相关分析显示端粒酶活性与hTERT蛋白表达存在显著相关性(P<0.05)。结论:端粒的短缩及端粒酶的活化与大肠癌的发生发展密切相关,hTERT的表达对端粒酶的激活可能起着重要作用。Purpose：To explore the role of terminal restriction fragments（TRFs）.telomerase activity and expression of human telomerase reverse transcriptase（hTERT） in colorectal carcinoma.Methods：The telomere length,telomerase activity and expression of hTERT wrer studied with Southern-blot. TRAP and immunohistochemistry,respectively.Results：TRFs in eancer tissue was much shorter than in the adjacent tissues and normal mucosa.and TRFs was decreased tgradually along with the development in cancer stage.The expression of Telomenase in colorecal carcinoma tissue was signifieantly higher than that in other tissues（P〈0.05）.Telomerase sctivity and expression of hTERT showed higher frequency in colorectal cartinoma with lymphatic metastasis.compared to those without lymphatic metastasis.Correlation alalysis revealed that expression of hTERT was significaltly related to that of telomerase activity（P〈0.05）.Conctusions：The occurrence and development of colorectal carcinoma may be closely related to telomere shorting and telomerase activation.HTERT expression probably plays a critical rode in telomerase activation.

关 键 词：大肠癌 端粒 端粒酶 端粒酶催化亚基 

分 类 号：R735.3[医药卫生—肿瘤]